Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture

BMC Microbiology
Nitar NweJimmy Kwang

Abstract

Prevention of a possible avian influenza pandemic necessitates the development of rapid diagnostic tests and the eventual production of a vaccine. For vaccine production, hemagglutinin (HA1) from avian influenza H5N1 was expressed from a recombinant baculovirus. Recombinant HA1 was expressed in monolayer or suspension culture insect cells by infection with the recombinant baculovirus. The yield of rHA1 from the suspension culture was 68 mg/l, compared to 6 mg/l from the monolayer culture. Immunization of guinea pigs with 50 microg of rHA1 yielded hemagglutinin inhibition and virus neutralization titers of 1:160 after two times vaccination with rHA1 protein. Thus, the production of rHA1 using an insect suspension cell system provides a promising basis for economical production of a H5 antigen.

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Citations

Nov 27, 2010·Analytical and Bioanalytical Chemistry·Scott A MillerDavid E Cliffel
Sep 24, 2014·Applied Biochemistry and Biotechnology·Arturo Sánchez-PazFernando Mendoza-Cano
Mar 17, 2007·Nature Reviews. Immunology·Kanta Subbarao, Tomy Joseph
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Mar 28, 2009·Biochemical and Biophysical Research Communications·Fang-Feng ChiuChih-Hsiang Leng

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Methods Mentioned

BETA
glycosylation
electrophoresis
protein assay

Software Mentioned

Quantity One

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