Expression of hepatitis C virus envelope protein 2 induces apoptosis in cultured mammalian cells

World Journal of Gastroenterology : WJG
Li-Xin ZhuYuan Wang

Abstract

To explore the role of hepatitis C virus (HCV) envelope protein 2 (E2) in the induction of apoptosis. A carboxyterminal truncated E2 (E2-661) was transiently expressed in several cultured mammalian cell lines or stably expressed in Chinese hamster ovary (CHO) cell line. Cell proliferation was assessed by 3H thymidine uptake. Apoptosis was examined by Hoechst 33258 staining, flow cytometry and DNA fragmentation analysis. Reduced proliferation was readily observed in the E2-661 expressing cells. These cells manifested the typical features of apoptosis, including cell shrinkage, chromatin condensation and hypodiploid genomic DNA content. Similar apoptotic cell death was observed in an E2-661 stably expressing cell line. HCV E2 can induce apoptosis in cultured mammalian cells.

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Citations

May 10, 2006·Biochemical and Biophysical Research Communications·Hui-Ling ChiouTzy-Yen Chen
Dec 3, 2009·Virology Journal·Christoph P BergGerburg M Stein
Jun 1, 2016·PloS One·Ednelza da Silva Graça AmorasAntonio Carlos Rosário Vallinoto
Aug 5, 2006·Pharmacogenomics·Yuchi HwangDing-Shinn Chen
Jul 12, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shiu-Wan Chan, Philip Anthony Egan
Nov 20, 2016·The Journal of Clinical Endocrinology and Metabolism·Sara Salehi HammerstadYaron Tomer

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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