Expression of heterobispecific antibodies by genes transfected into producer hybridoma cells

Gene
H Lenz, U H Weidle

Abstract

We report the expression of heterobispecific antibodies (Ab) by transferred genes. The kappa and gamma 1 genes of a mouse anti-idiotypic Ab (IgG1) were transfected into a mouse hybridoma cell line secreting Ab (IgG1), directed against an isoenzyme of human creatine kinase. Stable cell lines secreting the parental Ab derived from the introduced genes and a mixture of hybrid Ab were established. The transfected Ab specificity was expressed at similar levels as in a nonproducer background (50 ng-1 microgram/ml), heterobispecific Ab was expressed in microgram quantities (1-4 micrograms/ml) in all cell lines examined. As shown by isoelectric focusing analysis, hybrid Ab (heterobifunctional and other species) are expressed to a similar extent in the transfected cell lines as the Ab in the parental Ab-producing cells.

References

Jan 1, 1978·Annual Review of Microbiology·J Davies, D I Smith
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·W E Wright, L Hayflick
Jan 6, 1989·Science·E HaberM S Runge
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·U D Staerz, M J Bevan
Jan 1, 1987·European Journal of Immunology·A Lanzavecchia, D Scheidegger
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L K GillilandH Waldmann
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C Kocks, K Rajewsky
Apr 1, 1987·European Journal of Immunology·U D StaerzM J Bevan
Oct 6, 1983·Nature·C Milstein, A C Cuello
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A OchiN Hozumi
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P D BurrowsM R Wabl
Dec 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·E H SZYBALSKA, W SZYBALSKI

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.