Expression of HIV-1 accessory protein Vif is controlled uniquely to be low and optimal by proteasome degradation

Microbes and Infection
Mikako FujitaAkio Adachi

Abstract

While the Vif protein of human immunodeficiency virus type 1 (HIV-1) is essential for viral replication in non-permissive cells, it is rapidly degraded intracellularly. We have previously suggested that the rapid turn-over of Vif is biologically meaningful to prevent detrimental effects of this protein at high expression levels. We now studied the mechanism of Vif degradation by examining the blocking effect of protease inhibitors in pulse/chase experiments and by monitoring the extent of Vif ubiquitination. The rapid turn-over of Vif could be blocked by proteasome inhibitors, and Vif was highly ubiquitinated. Cytoskeletal Vif was found to be more stable than soluble cytosolic Vif. These degradation characteristics of Vif were cell type-independent and observed in both non-permissive and permissive cells. Characterization of a series of vif deletion mutants showed that amino acids predicted to be important for formation of beta-strand structures (amino acid nos. 63-70 and 86-89) were critical for maintaining a normal expression level of Vif and for viral infectivity. Finally, we performed comparative stability analysis of the four HIV-1 accessory proteins. Vif was unique in its short half-life and in the magnitude of the degrad...Continue Reading

Citations

Mar 24, 2005·Microbes and Infection·Huaqing WangMikako Fujita
Mar 3, 2007·AIDS Research and Human Retroviruses·Catherine M BellMaria A Papathanasopoulos
Sep 22, 2006·Viral Immunology·Mingzhou Chen, Denis Gerlier
Feb 12, 2010·Journal of Virology·Qiujia ShaoBindong Liu
Jan 11, 2007·Molekuliarnaia biologiia·E S StarodubovaV L Karpov
Jun 26, 2008·Retrovirology·Ritu Goila-Gaur, Klaus Strebel
Jan 9, 2009·Retrovirology·Taisuke IzumiTakashi Uchiyama
Feb 15, 2013·Chemical Biology & Drug Design·Wenlin HuangLihe Zhang
Sep 13, 2005·Microbes and Infection·Boonruang KhamsriAkio Adachi
Jan 15, 2014·Virology·Yusuke MatsuiAkifumi Takaori-Kondo
Oct 21, 2016·The Journal of Biological Chemistry·Yusuke MatsuiAkifumi Takaori-Kondo
Dec 14, 2018·Frontiers in Microbiology·Sneh LataAkhil C Banerjea
Mar 13, 2017·The Journal of General Virology·Minami YamamotoMikako Fujita
Nov 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wataru ShidaTakashi Watanabe
Jun 30, 2019·International Journal of Molecular Sciences·Vivian K Rojas, In-Woo Park
Dec 4, 2020·Microorganisms·Wendy Kaichun XuJaquelin P Dudley
Oct 12, 2021·Frontiers in Immunology·Wenying GaoXiaofang Yu
Nov 2, 2021·Chemical & Pharmaceutical Bulletin·Hiroshi TateishiMasami Otsuka

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