Expression of housekeeping and immunoproteasome subunit genes is differentially regulated in positively and negatively selecting thymic stroma subsets

European Journal of Immunology
Ahmed NilGabriele Niedermann

Abstract

The expression of housekeeping and/or immunoproteasomes in isolated thymic stroma subsets has so far not been analyzed but may have important consequences for self peptide repertoires presented by MHC class I molecules during positive and negative thymic selection. Here we determined the expression of housekeeping and immunoproteasome beta subunits and of PA28 in positively and negatively selecting stroma subsets. Positively selecting cortical thymic epithelial cells (cTEC) expressed only housekeeping but no immunoproteasome beta subunit mRNA and proteins. However, immunoproteasome beta subunits could be induced in cTEC by infection with Listeria monocytogenes or injection of IFN-gamma. In negatively selecting stroma including medullary epithelial cells and dendritic cells, incomplete and low representation of housekeeping beta subunit proteins but high and complete expression of immunoproteasome beta subunit proteins suggests absence of proper housekeeping proteasomes and predominance of immunoproteasomes. Expression of immunoproteasome beta subunits in negatively selecting stroma was independent of IFN-gamma receptor as shown in knockout (KO) mice. Absence of LMP2 altered thymic selection of the MHC class I-restricted transge...Continue Reading

Citations

May 7, 2013·Archivum Immunologiae Et Therapiae Experimentalis·Utano Tomaru, Masanori Kasahara
Nov 30, 2013·Trends in Biochemical Sciences·Andrea Kniepert, Marcus Groettrup
Dec 27, 2011·Nature Immunology·Eleanor Z KincaidKenneth L Rock
Jun 29, 2005·The Journal of Experimental Medicine·Jens DerbinskiBruno Kyewski
May 7, 2008·Biochemistry. Biokhimii︠a︡·V I MelnikovaL A Zakharova
Mar 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Philipp OsterlohHansjörg Schild
Jul 18, 2014·Biomolecules·Minghui BaiShigeo Murata
Oct 28, 2008·Seminars in Immunology·Michelle A NellerChristopher W Schmidt
Sep 16, 2008·Biochimica Et Biophysica Acta·Markus Frericks, Charlotte Esser
Apr 12, 2008·Current Opinion in Immunology·Shigeo MurataKeiji Tanaka
Apr 23, 2009·Journal of Internal Medicine·G A Holländer, P Peterson
Dec 25, 2010·European Journal of Immunology·Alexandre DaletBenoît J Van den Eynde
Sep 14, 2010·Current Opinion in Immunology·Jens Derbinski, Bruno Kyewski
Aug 4, 2006·Cellular Immunology·Theodoros EleftheriadisGrammatiki Galaktidou
Jul 15, 2015·BioMed Research International·Nathalie Vigneron
Dec 31, 2011·Current Opinion in Immunology·Nathalie Vigneron, Benoît J Van den Eynde
Jan 23, 2015·Frontiers in Pharmacology·Andrea I LoewendorfAlan Flake
May 9, 2018·Immunology and Cell Biology·Damien ZankerWeisan Chen
Dec 17, 2009·Nature Reviews. Immunology·Marcus GroettrupMichael Basler
Feb 17, 2010·Immune Network·Kwon Ik Oh, Jae Nam Seo
May 8, 2019·International Journal of Molecular Sciences·Fiona LimanaqiFrancesco Fornai
Mar 28, 2019·The Journal of Biological Chemistry·Ulrike KuckelkornJuliane Liepe
Jan 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jacques ChapiroBenoît J Van den Eynde
Mar 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christy M CaudillThomas A Griffin
Oct 27, 2017·European Journal of Immunology·Michael BaslerMarcus Groettrup
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alexander K NussbaumJ Lindsay Whitton
Nov 23, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ken C PangWeisan Chen
Jul 10, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael BaslerMarcus Groettrup
Jun 8, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael BaslerChristopher J Kirk
May 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael BaslerJohn J Monaco
Apr 16, 2019·Frontiers in Immunology·Fiona LimanaqiFrancesco Fornai
Oct 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Britta StrehlUlrich Steinhoff
Feb 9, 2021·The Journal of Experimental Medicine·Izumi OhigashiYousuke Takahama
Mar 4, 2021·Pathology International·Masanori Kasahara
Jun 2, 2007·Science·Michael J Bevan
Oct 5, 2010·Cellular Immunology·Victoria I MelnikovaLiudmila A Zakharova

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