PMID: 11912063Mar 26, 2002Paper

Expression of HSP70 and CYP1A protein in ovary and liver of juvenile rainbow trout exposed to beta-naphthoflavone

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
Lynn P WeberD M Janz

Abstract

Cytochrome P4501A (CYP1A) and the 70-kDa stress protein (HSP70) were determined using Western blotting in the ovary and liver of juvenile female rainbow trout (Oncorhynchus mykiss) exposed for 4 days to beta-naphthoflavone (betaNF) following a single intraperitoneal injection. Ovarian CYP1A protein was observed in both control and betaNF-exposed fish, indicating constitutive and inducible expression of CYP1A in immature trout ovaries. CYP1A protein levels determined using densitometry were 14- and 46-fold greater in betaNF-exposed trout compared to controls in the liver and ovary, respectively. Hepatic microsomal ethoxyresorufin O-deethylase (EROD) activity, a specific catalytic marker of CYP1A, was also induced 38-fold above controls following betaNF exposure. Hepatic HSP70 protein expression was significantly higher in whole cell homogenates, but not in cytosolic fractions, collected from betaNF-exposed fish in comparison to control fish. There was no difference in ovarian HSP70 levels determined in whole cell homogenates between control and betaNF-exposed fish. The observation that unlike liver, ovarian HSP70 expression remained unchanged following induction of CYP1A protein may be related to the sensitivity of the teleost o...Continue Reading

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Citations

Apr 6, 2004·Aquatic Toxicology·Neelakanteswar Aluru, Mathilakath M Vijayan
Nov 2, 2005·Tissue Engineering·Padmavathy RajagopalanMartin L Yarmush
Sep 2, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hélène RimeGilles Monod
Jul 10, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Daniela NestlerPaola Pocar
May 19, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Meagan E Gourley, Christopher J Kennedy
Apr 15, 2010·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Julian Rios-SicairosAlejandra Garcia-Gasca
May 21, 2020·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Thays de Andrade GuedesCarmem Silvia Fontanetti
Oct 21, 2016·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Yadira Ansoar-RodríguezCarmem S Fontanetti
Apr 20, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ahmad N Al-Dissi, Lynn P Weber

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