PMID: 107601Jan 1, 1979

Expression of human hepatic genes in mouse hepatoma--human amniocyte hybrids

Somatic Cell Genetics
J K Rankin, G J Darlington

Abstract

Human liver-specific gene products are expressed by hybrid cells resulting from the fusion of human amniocytes with mouse hepatoma cells. Amniocytes grown from human amniotic fluid have no detectable levels of secreted human albumin, transferrin, alpha-1 antitrypsin, or ceruloplasmin, while the mouse hepatoma line, HH--, secretes several mouse liver-specific gene products including transferrin and albumin. Fifty-five hybrids were isolated and analyzed for the expression of serum proteins by Ouchterlony double diffusion and Laurell immunoelectrophoresis. All hybrids continued to express mouse albumin and transferrin, and 29 hybrids from this series were found to express one or more human serum proteins. Activation of the human amniocyte genome provides a model for prenatal diagnosis of serum protein abnormalities.

References

Oct 16, 1975·Differentiation; Research in Biological Diversity·C Szpirer, J Szpirer
Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·F GilbertF H Ruddle
Oct 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·E B Thompson, T D Gelehrter
Aug 1, 1973·Biochemical Genetics·D RintoulJ Morrow
Mar 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J A Peterson, M C Weiss
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R S Sparkes, M C Weiss
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·S E Malawista, M C Weiss
Jan 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J A Schneider, M C Weiss
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Jul 15, 1973·American Journal of Obstetrics and Gynecology·L L CederqvistD J Rothe

Citations

Jan 1, 1980·Somatic Cell Genetics·R HalabanJ M Eisenstadt
May 1, 1982·Somatic Cell Genetics·G J DarlingtonG Schlanger
Aug 1, 1989·Trends in Genetics : TIG·H M Blau
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T V Gopalakrishnan, W F Anderson
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G J DarlingtonM Smith
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·W J RettigL J Old
Dec 15, 1982·Biochemical and Biophysical Research Communications·C FinazJ M Saez
Jul 1, 1986·Toxicology and Applied Pharmacology·E P SeravalliJ E Cottrell
Aug 12, 1999·Seminars in Cell & Developmental Biology·H M Blau, B T Blakely

Related Concepts

Zemaira
Structure of Amnion
Ferroxidase I
Immunodiffusion Measurement
Cistron
Hybrid Cells
Immunoelectrophoresis
Liver
Hepatoma, Novikoff
Plasma Albumin

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