PMID: 2499113Jun 1, 1989Paper

Expression of human immunodeficiency virus type 1 (HIV-1) gag antigens on the surface of a cell line persistently infected with HIV-1 that highly expresses HIV-1 antigens

Virology
K IkutaS Kato

Abstract

MT-4 cells persistently infected with human immunodeficiency virus type 1 (HIV-1) (MT-4/HIV-1) were recently isolated (K. Ikuta, C. Morita, M. Nakai, N. Yamamoto, and S. Kato, Japan. J. Cancer Res. (Gann), 79, 418-423, 1988). Mouse hybridoma cell clones producing monoclonal antibodies (MoAbs) to HIV-1 gag p24 and p18, and pol reverse transcriptase (RT) were isolated by using this MT-4/HIV-1 cell line for the screening of MoAb production by the immunofluorescence (IF) test. By indirect IF tests of acetone-fixed cells with these MoAbs, the IF intensities in MT-4/HIV-1 cells were found to be higher than those in the other HIV-1 infected cells, such as MOLT-4/HIV-1, HL-60/HIV-1, and U937/HIV-1 cells. Cell surface expression of the HIV-1 gag p24 and p18 antigens examined by IF and radioimmune techniques with these MoAbs revealed the p24 and p18 antigens to be expressed strongly on the cell surface of MT-4/HIV-1 cells and faintly on the cell surface of MOLT-4/HIV-1 cells, respectively. However, monoclonal antibody isolated in the present study failed to detect pol RT antigen on the surface of MT-4/HIV-1 cells. These results indicate that the gag p24 and p18 antigens are expressed, at least in part, on the surface of HIV-1-infected ce...Continue Reading

References

May 15, 1976·International Journal of Cancer. Journal International Du Cancer·C Sundström, K Nilsson
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S J CollinsR C Gallo
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F di Marzo VeroneseR C Gallo
Jul 4, 1986·Cell·J M Coffin
Jan 1, 1986·Medical Microbiology and Immunology·H NakashimaN Yamamoto
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H K LyerlyK J Weinhold
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D A Thorley-Lawson, E S Israelsohn
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A S Fauci
Dec 1, 1987·The Journal of Infectious Diseases·R S BlumbergR T Schooley
Jul 23, 1987·Nature·B D WalkerR T Schooley

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Citations

Feb 15, 2001·Journal of Medical Virology·T GotoK Sano
Jun 1, 1992·Journal of Medical Virology·L FrankeR von Baehr
May 1, 1995·Veterinary Immunology and Immunopathology·D MatteucciM Bendinelli
Jul 31, 1998·Micron : the International Research and Review Journal for Microscopy·T GotoK Ikuta
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A AchourA L Goldstein
Jul 1, 1995·The Journal of Experimental Medicine·Q J Sattentau, J P Moore
Mar 1, 1991·AIDS Research and Human Retroviruses·R B FernsR S Tedder
Apr 10, 1996·AIDS Research and Human Retroviruses·B JanvierF Barin
Jan 1, 1992·Microbiology and Immunology·A SaitohH Shinagawa
Jun 27, 2007·FEBS Letters·Tat San LauDavid Chi Cheong Wan
Jan 22, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Mika Nagai-FukatakiHisatoshi Shida
Oct 19, 2010·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Manju BalaKazuyoshi Ikuta

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