Expression of human oocyte-specific linker histone protein and its incorporation into sperm chromatin during fertilization

Fertility and Sterility
Yuri MizusawaYasunori Yoshimura

Abstract

To investigate the expression of oocyte-specific linker histone protein (hH1FOO) in human ovaries and its incorporation into sperm chromatin after intracytoplasmic sperm injection (ICSI). Laboratory study. University hospital. Human ovarian tissues were obtained from patients at oophorectomy. Human oocytes and embryos were obtained from infertile patients undergoing IVF and ICSI. A polyclonal rabbit antibody targeting the predicted hH1FOO protein was used for immunohistochemical analysis. Western blot analysis and the reverse transcriptase-nested polymerase chain reaction were done to detect hH1FOO in chromatin of germinal vesicle-stage oocytes through to two-cell embryos. The hH1FOO antibody reactivity of oocytes, ovarian tissues, and sperm chromatin after ICSI. hH1FOO protein was localized in all oocytes from primordial to Graafian follicles. In unfertilized oocytes after ICSI, the chromatin of injected sperm was condensed without hH1FOO incorporation in 45.5% of oocytes, condensed with hH1FOO incorporation in 9%, and decondensed with hH1FOO incorporation in 45.5%. None of the oocytes contained decondensed sperm chromatin without hHFOO incorporation. hH1FOO protein was expressed by human oocytes from primordial follicles to e...Continue Reading

References

Jan 1, 1991·Molecular Reproduction and Development·P G AdenotP Debey
Jan 1, 1990·Molecular Reproduction and Development·S Nonchev, R Tsanev
Jan 1, 1995·Reproduction, Fertility, and Development·S P FlahertyC D Matthews
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·H Kandolf
Jul 27, 2001·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M H Parseghian, B A Hamkalo
Oct 4, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·Y NakazawaK Kikuchi
Apr 25, 2003·Biochemical and Biophysical Research Communications·Yudai TanakaYasunori Yoshimura
Apr 26, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·David W McLay, Hugh J Clarke
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Raouf AlamiEric E Bouhassira
Sep 17, 2004·Biology of Reproduction·Mamoru TanakaEli Y Adashi
Dec 27, 2005·Cell·Ralph A W Rupp, Peter B Becker
Feb 14, 2006·Molecular Reproduction and Development·Serge McGrawMarc-André Sirard
Mar 4, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Michiko NakaiKazuhiro Kikuchi
Jun 15, 2006·Briefings in Functional Genomics & Proteomics·Juan Ausió

❮ Previous
Next ❯

Citations

May 2, 2014·Cell and Tissue Research·Burcu Biterge, Robert Schneider
Apr 12, 2013·Reproduction in Domestic Animals = Zuchthygiene·I R BessaM A N Dode
Mar 13, 2014·Zygote : the Biology of Gametes and Early Embryos·Yan YunAn-Min Lei
Apr 17, 2016·Nucleic Acids Research·Yair AaronsonEran Meshorer
Apr 30, 2015·Chromosoma·Salvador Pérez-MonteroFernando Azorín
Jul 22, 2018·Scientific Reports·Eva ŠatovićFernando Azorín
Jan 18, 2017·Epigenetics & Chromatin·Sara El KennaniJérôme Govin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.