PMID: 9524070Apr 2, 1998Paper

Expression of human pro-matrix metalloproteinase 3 that lacks the N-terminal 34 residues in Escherichia coli: autoactivation and interaction with tissue inhibitor of metalloproteinase 1 (TIMP-1)

Biological Chemistry
K SuzukiHideaki Nagase

Abstract

Human pro-matrix metalloproteinase 3 (proMMP-3) lacking the N-terminal 34 amino acids and the C-terminal hemopexin-like domain was expressed in E. coli and used to investigate the process of proenzyme activation and its interaction with an endogenous inhibitor TIMP-1 during activation. The truncated precursor was purified from the E. coli extract in the presence of 5mM EGTA. The active 23.5 kDa form was generated simply by exposure to Ca2+ and Zn2+ but not either by Ca2+ alone or by Zn2+ alone. The rate of MMP-3(deltaC) formation was concentration dependent, indicating that autoactivation is a bimolecular reaction. The truncated precursor was able to interact with the N-terminal domain of TIMP-1 without losing the 48 residue-long propeptide. However, upon a longer incubation, the propeptide was slowly processed, indicating that the association of the N-terminally truncated proMMP-3 with TIMP-1 is weaker than that of the fully activated MMP-3 and TIMP-1. These results indicate that the expression of MMP activities is regulated by endogenous inhibitor TIMPs during their activation processes which provide an additional control mechanism of extracellular matrix breakdown.

References

Jan 1, 1995·Methods in Enzymology·H Nagase
Jan 1, 1995·Methods in Enzymology·G Murphy, F Willenbrock
Oct 1, 1995·Protein Science : a Publication of the Protein Society·J W BeckerJ D Hermes

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Citations

Mar 26, 2003·Protein Expression and Purification·Oscar H P RamosHeloisa S Selistre-de-Araujo
Aug 4, 2007·Journal of the American Chemical Society·Janelle Lauer-FieldsGregg B Fields
Jul 4, 2009·The Journal of Biological Chemistry·Janelle L Lauer-FieldsGregg B Fields
May 24, 2008·The Journal of Biological Chemistry·Janelle L Lauer-FieldsGregg B Fields
Jul 26, 2011·Biochimica Et Biophysica Acta·Nicole HoweJ Paul G Malthouse
Jun 21, 2011·European Journal of Cell Biology·Heiko MeyerAchim Paululat
Aug 1, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·Linda TroebergHideaki Nagase
Mar 22, 2008·Biochemical and Biophysical Research Communications·Yunge ZhaoIsa M Hussaini
Sep 5, 2003·Osteoarthritis and Cartilage·K BobaczW B Graninger
Feb 15, 2019·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Ursula MirastschijskiSørge Kelm
Mar 21, 2001·Biotechnology & Genetic Engineering Reviews·L M BabéB F Schmidt
May 2, 2000·The Journal of Biological Chemistry·J L Lauer-FieldsG B Fields
Mar 30, 2001·The Journal of Biological Chemistry·M KashiwagiK Brew

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