Expression of IL-8, IL-6 and IL-1β in tears as a main characteristic of the immune response in human microbial keratitis

International Journal of Molecular Sciences
Concepcion SantacruzMaria C Jimenez-Martinez

Abstract

Corneal infections are frequent and potentially vision-threatening diseases, and despite the significance of the immunological response in animal models of microbial keratitis (MK), it remains unclear in humans. The aim of this study was to describe the cytokine profile of tears in patients with MK. Characteristics of ocular lesions such as size of the epithelial defect, stromal infiltration, and hypopyon were analyzed. Immunological evaluation included determination of interleukine (IL)-1β, IL-6, IL-8, IL-10, IL-12 and tumor necrosis factor (TNF)-α in tear samples obtained from infected eyes of 28 patients with MK and compared with their contralateral non-infected eyes. Additionally, frequency of CD4+, CD8+, CD19+ and CD3-CD56+ cells was also determined in peripheral blood mononuclear cells in patients with MK, and compared with 48 healthy controls. Non-significant differences were observed in the size of the epithelial defect, stromal infiltration, and hypopyon. Nevertheless, we found an immunological profile apparently related to MK etiology. IL-8 > IL-6 in patients with bacterial keratitis; IL-8 > IL-6 > IL-1β and increased frequency of circulating CD3-CD56+ NK cells in patients with gram-negative keratitis; and IL-8 = IL-6...Continue Reading

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Citations

Mar 15, 2016·Biomedit︠s︡inskai︠a︡ khimii︠a︡·A E Grigor'evaE I Ryabchikova
Jul 10, 2019·Scientific Reports·Xiaohua LiShikun He
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Oct 19, 2017·Scientific Reports·Jae Hun JungKwang Pyo Kim
Oct 30, 2020·Frontiers in Immunology·Vladimir Rogovskii
Mar 19, 2021·Ocular Immunology and Inflammation·Alireza ShahriaryHossein Aghamollaei
Mar 28, 2021·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Parisa MirzapourLyndon Jones

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Methods Mentioned

BETA
lavage
flow cytometry

Software Mentioned

GraphPad
CellQuest Pro
BD Cytometric bead array

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