Expression of IMPDH1 and IMPDH2 after transplantation and initiation of immunosuppression

Transplantation
Sara BremerStein Bergan

Abstract

Mycophenolic acid (MPA) mediates immunosuppressive effects by inhibiting inosine monophosphate dehydrogenase (IMPDH). Induction of IMPDH activity has been observed in whole blood and erythrocyte samples during immunosuppressive therapy. Information concerning the mechanisms for increased IMPDH activity is limited and the potential implications of induction have been debated. Whole blood, CD4+ cell, and reticulocyte samples were collected from 30 renal transplant patients pre- and posttransplantation. The expressions of two IMPDH isoforms, type 1 and 2, were analyzed by real-time reverse-transcription polymerase chain reaction and quantified using a housekeeping gene index. The IMPDH activity was determined by ultraviolet high-performance liquid chromatography. Transplantation and the initiation of immunosuppressive therapy was associated with increased IMPDH1 (50-88%, P<0.0005) and decreased IMPDH2 (42-56%, P<0.0005) expression. In CD4+ cells, however, IMPDH2 increased (15%, P=0.009). These changes are probably related to glucocorticoid effects. Two weeks posttransplant, MPA-treated patients displayed elevated IMPDH 1 and 2 in reticulocytes, suggesting enzyme induction in these cells during prolonged MPA therapy. Patients with ...Continue Reading

References

Nov 1, 1991·Molecular and Cellular Biology·D A GlesneE Huberman
Feb 13, 1999·Kidney International·L C RacusenY Yamaguchi
Aug 23, 2000·The Journal of Clinical Investigation·J J GuB S Mitchell
Oct 18, 2000·The Journal of Biological Chemistry·M Escobar-Henriques, B Daignan-Fornier
Dec 14, 2002·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Kevin C MangeHarold I Feldman
Aug 29, 2003·Molecular and Cellular Biology·Jing Jin GuBeverly S Mitchell
Jan 6, 2004·International Journal of Clinical Pharmacology and Therapeutics·P GlanderK Budde
Dec 4, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Petra GlanderKlemens Budde
Jan 7, 2005·Clinical Pharmacokinetics·David CzockUlla Häussler
Jan 7, 2006·Journal of the American Society of Nephrology : JASN·Holger AndreePetra Reinke
Feb 9, 2006·Scandinavian Journal of Clinical and Laboratory Investigation·N T VetheS Bergan
Mar 29, 2007·Journal of the American Society of Nephrology : JASN·Joshua J AugustineDonald E Hricik
Apr 28, 2007·Clinical Chemistry·Sara BremerStein Bergan

❮ Previous
Next ❯

Citations

Dec 12, 2012·Pharmacogenomics and Personalized Medicine·Hamid Abboudi, Iain Am Macphee
Feb 9, 2010·Pharmacogenomics·Gilbert J Burckart, Shashi Amur
May 6, 2014·Archives of Toxicology·Christine E Staatz, Susan E Tett
Nov 18, 2008·International Immunopharmacology·Sara BremerStein Bergan
Oct 11, 2015·Clinical Biochemistry·Stein BerganNils Tore Vethe
Jan 19, 2010·British Journal of Clinical Pharmacology·Laurent R ChiarelliMario Regazzi
Mar 12, 2015·Transplant International : Official Journal of the European Society for Organ Transplantation·Teun van Gelder, Dennis A Hesselink
Dec 20, 2013·Drug Metabolism and Pharmacokinetics·Min DongAlexander A Vinks
Oct 9, 2009·International Reviews of Immunology·Volker Daniel, Gerhard Opelz
Apr 2, 2010·Journal of Sex Research·Monica M Moore
Jul 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Li-Xi LiaoPeng-Fei Tu
Sep 15, 2017·Nephrology·Tom Lea-Henry, Bobby Chacko

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.