Expression of indoleamine 2,3-dioxygenase in nasopharyngeal carcinoma impairs the cytolytic function of peripheral blood lymphocytes.

BMC Cancer
Peng LiuJun Du

Abstract

Tumor-specific cytotoxic T cells and infiltrating lymphocytes are frequently found in tumor tissues in patients with nasopharyngeal carcinoma (NPC). Most patients with NPC, however, especially those with advanced stages, have a poor clinical prognosis despite conventional immunotherapy. The aim of this work was to examine the effect of indoleamine 2,3-dioxygenase (IDO), an immunosuppressive enzyme, on the lymphocyte function in NPC. The NPC cell line CNE2 was treated by interferon-gamma (IFNgamma) and the levels of IDO expression was analyzed by Western blotting and reverse phase high-performance liquid chromatography (HPLC). Lymphocytes from health human exposed to the milieu created by IDO-positive CNE2 cells and the lymphocyte cytotoxicity to target tumor cells was analyzed by standard lactate dehydrogenase (LDH) release assay. Additionally, expression of IDO was determined by Immunohistochemical assay in the tumor tissues form clinically evaluated NPC. IDO expression was acutely induced in the NPC cell line CNE2 by low dose interferon-gamma (IFNgamma) or by co-incubation with activated lymphocytes. Exposure to the milieu created by IDO-positive CNE2 cells did not promote lymphocyte death, but lymphocyte cytotoxicity against...Continue Reading

References

Jan 1, 1997·Annual Review of Immunology·U BoehmJ C Howard
May 4, 1999·The Journal of Experimental Medicine·D H MunnA L Mellor
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·P HwuH A Young
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew L MellorDavid H Munn
Aug 6, 2002·Journal of Neuroimmunology·Kenichi SakuraiGene M Shearer
Sep 17, 2002·Cell Death and Differentiation·F FallarinoP Puccetti
Oct 9, 2002·Nature Immunology·Ursula GrohmannPaolo Puccetti
May 10, 2003·Trends in Immunology·Ursula GrohmannPaolo Puccetti
Aug 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ursula GrohmannPaolo Puccetti
Oct 3, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J-P SpanoJ-P Armand
Mar 23, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·David H MunnAndrew L Mellor
Apr 8, 2004·Current Opinion in Oncology·Sandrine FaivreJean-Pierre Armand
Jul 16, 2004·The Journal of Clinical Investigation·David H MunnAndrew L Mellor
Oct 2, 2004·Nature Reviews. Immunology·Andrew L Mellor, David H Munn
Jun 14, 2005·Lancet·William I Wei, Jonathan S T Sham
Aug 24, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Aikou OkamotoMitsuyoshi Urashima
Oct 20, 2005·Clinical and Experimental Immunology·J EnckeW Stremmel
Feb 21, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Gerald BrandacherAlbert Amberger
Oct 6, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kenneth W YipFei-Fei Liu
Nov 23, 2006·British Journal of Cancer·K InoF Kikkawa
Feb 23, 2007·International Journal of Cancer. Journal International Du Cancer·Takeshi OginoSoldano Ferrone
May 31, 2007·The Journal of Biological Chemistry·Shane R ThomasRoland Stocker
Oct 3, 2007·Journal of Cancer Research and Clinical Oncology·Xueling OuJun Du
Mar 4, 2008·International Journal of Immunogenetics·K FarhatL Chouchane
May 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Burles A JohnsonAndrew L Mellor

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Citations

Aug 10, 2010·The International Journal of Biochemistry & Cell Biology·Guan-Min JiangJun Du
Aug 21, 2018·Annual Review of Pathology·Paul J Farrell
Jul 6, 2020·International Immunology·Alok Kumar, Kenji Chamoto
Nov 10, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jessica Godin-EthierRéjean Lapointe
Feb 10, 2021·Journal of Experimental & Clinical Cancer Research : CR·Yu YaoQing Yang

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Methods Mentioned

BETA
density gradient centrifugation
electrophoresis
FCS
Assay

Software Mentioned

Breeze

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