Expression of insect α6-like nicotinic acetylcholine receptors in Drosophila melanogaster highlights a high level of conservation of the receptor:spinosyn interaction

Insect Biochemistry and Molecular Biology
Trent PerryPhilip Batterham

Abstract

Insecticide research has often relied on model species for elucidating the resistance mechanisms present in the targeted pests. The accuracy and applicability of extrapolations of these laboratory findings to field conditions varies but, for target site resistance, conserved mechanisms are generally the rule rather than the exception (Perry et al., 2011). The spinosyn class of insecticides appear to fit this paradigm and are a pest control option with many uses in both crop and animal protection. Resistance to spinosyns has been identified in both laboratory-selected and field-collected pest insects. Studies using the model insect, Drosophila melanogaster, have identified the nicotinic acetylcholine receptor subunit, Dα6 as an important target of the insecticide spinosad (Perry et al., 2007; Watson et al., 2010). Field-isolated resistant strains of several agricultural pest insects provide evidence that resistance cases are often associated with mutations in orthologues to Dα6 (Baxter et al., 2010; Puinean et al., 2013). The expression of these receptors is difficult in heterologous systems. In order to examine the biology of the Dα6 receptor subunit further, we used Drosophila as a model and developed an in vivo rescue system....Continue Reading

References

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Mar 24, 2011·Insect Biochemistry and Molecular Biology·Trent PerryPhillip J Daborn
Sep 2, 2014·Pesticide Biochemistry and Physiology·Grant A HerronCarlotta Gobbi
Oct 17, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Minoru NakayamaChihiro Hama

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Citations

Nov 3, 2016·Journal of Neurogenetics·Richard H Ffrench-ConstantChris Bass
Jun 26, 2015·Nature Communications·Clare A AnsteadRobin B Gasser
May 21, 2020·Pest Management Science·Ana Guillem-AmatFélix Ortego
Jun 23, 2020·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Guilu Zhang, Wenjun Zhang
Nov 18, 2020·Pest Management Science·Jianqiang LanQian Han

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