Expression of Insulin-Like Growth Factor Binding Protein-5 (IGFBP5 ) Reverses Cisplatin-Resistance in Esophageal Carcinoma

Cells
Dessy ChanJohnny C O Tang

Abstract

Cisplatin (CDDP) is one of the front-line chemotherapeutic drugs used in the treatment of esophageal squamous cell carcinoma (ESCC). Occurrence of resistance to CDDP has become one of the main challenges in cancer therapy. In this study, the gene expression profile of CDDP-resistant ESCC cells was investigated and molecular approaches were explored in an attempt to reverse the CDDP resistance. A CDDP-resistant SLMT-1/CDDP1R cell line was established from SLMT-1 cells by subculturing in the medium containing an increasing concentration of CDDP (0.1⁻1μg/mL). Mitochondrial (MTS) cytotoxicity assay, cell proliferation assay and cell morphology were used to assess the acquisition of cisplatin-resistance. The most differentially expressed gene in SLMT-1/CDDP1R cells was identified by cDNA microarray analysis compared with the parental SLMT-1 cells and validated by quantitative real-time polymerase chain reaction (qPCR). Association between expression of the most differentially expressed target gene to cisplatin-resistance was verified by RNA interference. An attempt to reversecisplatin-resistance phenotypes was made by using the vector expressing the most downregulated target gene in the CDDP-resistant cells. A CDDP-resistant ESCC ce...Continue Reading

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Citations

Jul 10, 2019·Acta Biochimica Et Biophysica Sinica·Zhenghua ZhangMingfa Guo
Aug 25, 2020·Cancer Cell International·Yongjun ZhuXiaofeng Chen
Mar 27, 2021·Frontiers in Cell and Developmental Biology·Soudeh Ghafouri-FardMohammad Taheri

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Methods Mentioned

BETA
transfection

Software Mentioned

Affymetrix
GraphPad
GraphPad Prism
Affymetrix GeneChip Operating Software

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