Expression of insulin-like growth factor-I and its receptor by SV40-transformed rat granulosa cells.

Molecular Endocrinology
M ZilbersteinK J Catt

Abstract

Cellular proliferation is a dominant aspect of ovarian follicular development in the rat, and insulin-like growth factor I (IGF-I) has been proposed as a mediator of cellular growth and differentiation in the ovary. An SV40-transformed rat granulosa cell line (RGA-41S) has been established as a model for studies on dividing cells of granulosa origin. Granulosa cells from the ovaries of immature diethylstilbestrol-treated rats were infected with the tsA255 mutant of SV40, followed by cloning in serum-free medium to select transformed cell lines which were serum independent. At the permissive temperature (33 C), RGA-41S cells exhibited a transformed phenotype and rapidly formed high density multilayers of compact cells that readily overgrew nontransformed cells. At the nonpermissive temperature (40 C) cell replication declined and division ceased after 4 days. Furthermore, at 40 C the cells grew as a monolayer and assumed a tetrahedral shape with a high cytoplasm-to-nucleus ratio, and displayed reduced ability to overgrow nontransformed cells. The transformed ovarian cells did not express detectable gonadotropin receptors and steroidogenic activity but retained their epithelial phenotype as demonstrated by cytokeratin staining of...Continue Reading

Citations

Jun 11, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·L S SteinR C Burghardt
Jul 1, 1993·Molecular and Cellular Endocrinology·I M RaoV B Mahesh
Nov 1, 1993·Molecular and Cellular Endocrinology·J K LeightonJ M Hammond
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Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H WernerD LeRoith
Nov 1, 1993·In Vitro Cellular & Developmental Biology. Animal·T W BriersH Vanderstichele
Nov 16, 2004·Molecular and Cellular Endocrinology·Jon C HavelockBruce R Carr
Jun 29, 1990·Biochemical and Biophysical Research Communications·H WernerC T Roberts

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