Expression of insulin-like growth factor-II and insulin-like growth factor binding proteins during Caco-2 cell proliferation and differentiation

Journal of Cellular Physiology
J H ParkR G MacDonald

Abstract

The components of the insulin-like growth factor (IGF) axis and their roles in regulating proliferation and differentiation of the human colon adenocarcinoma cell line, Caco-2, have been investigated. Caco-2 cells proliferated in serum-free medium at 75% the rate observed in medium containing 10% fetal bovine serum. IGF-I (10 nM) increased Caco-2 cell growth in serum-free medium, but not to the rate seen with serum. Multiple IGF-II mRNA species were produced by Caco-2 cells, but IGF-I mRNA was undetectable. Secretion of radioimmunoassayable IGF-II corresponded with steady-state levels of IGF-II mRNA, neither of which was observed to change markedly over the course of 16 days of Caco-2 cell differentiation. Levels of sucrase-isomaltase mRNA, a marker for enterocytic differentiation, increased 12-fold between days 5 and 16 of culture. Northern blotting of total RNA and ligand blot and immunoblot analyses of serum-free conditioned medium revealed that Caco-2 cells produce several IGF binding proteins (IGFBPs), including IGFBP-2, -3, and -4, as well as a 31,000 M(r) species that was not identified. The pattern of IGFBP secretion changed dramatically during Caco-2 cell differentiation: IGFBP-3 and IGFBP-2 increased 8.5-fold and 5-fo...Continue Reading

References

Jul 1, 1977·Journal of the National Cancer Institute·J FoghT Orfeo
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Gossen, H Bujard
Feb 1, 1991·The American Journal of Physiology·C ItoN Akaike
Dec 1, 1991·The American Journal of Physiology·H S Cross, A Quaroni
Feb 15, 1987·Analytical Biochemistry·L C Davis, G A Radke
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·A D Malcolm, G Snounou
Sep 1, 1984·The American Journal of Physiology·E GrassetJ F Desjeux
Apr 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P M SchiltzD J Baylink
Oct 29, 1993·Biochemical and Biophysical Research Communications·S OguchiI R Sanderson
Feb 1, 1995·Endocrine Reviews·J I Jones, D R Clemmons
Jan 1, 1994·Annual Review of Physiology·G S EvansC S Potten
Jan 1, 1993·Annual Review of Physiology·W S Cohick, D R Clemmons
Jan 1, 1964·Analytical Biochemistry·A DAHLQVIST

❮ Previous
Next ❯

Citations

Aug 7, 2002·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Mark R CorkinsRichard G MacDonald
Feb 24, 1999·Inflammatory Bowel Diseases·P L Beck, D K Podolsky
Jan 28, 1999·American Journal of Respiratory Cell and Molecular Biology·W A Price
Sep 14, 2000·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·L MishraL Korman
Jun 7, 2005·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Richard Makins, Anne Ballinger
Sep 30, 2004·International Journal of Cancer. Journal International Du Cancer·Daniela DiehlStefan Bauersachs
Apr 1, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Carmen Z MichayliraP Kay Lund
Jul 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Eun J KimJung H Y Park

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.