Expression of intercellular adhesion molecule-1 messenger ribonucleic acid and protein in human term placental cells and its modulation by pro-inflammatory cytokines (interleukin-1beta and tumor necrosis factor alpha)

Biology of Reproduction
B GaffuriM Vignali

Abstract

Intercellular adhesion molecule-1 (ICAM-1) is a ligand for the integrins lymphocyte function-associated antigen-1 (LFA-1) and complement receptor-3 (Mac-1), making it an important participant in many immune and inflammatory processes. Previous studies suggested that lack or reduced expression of ICAM-1 on trophoblast might partially explain its resistance to lysis by cytotoxic effectors. However, whether or not the adhesion molecule is expressed on placental cells is still a matter of debate. In this study, we determined ICAM-1 expression at mRNA, surface, and soluble protein levels on human trophoblasts throughout their functional differentiation in culture from cytotrophoblasts into syncytiotrophoblasts. Placental cells were obtained from 6 term placentas derived from normal pregnancies. ICAM-1 mRNA was detected by reverse transcription-polymerase chain reaction using two oligonucleotide primers specific for the human ICAM-1 gene. A single major DNA band of the expected size (943 base pairs) was obtained in both cytotrophoblasts and syncytiotrophoblasts. Flow cytometric analysis demonstrated expression of surface ICAM-1 protein on 45.5+/-3.5% of cytotrophoblasts. No changes were observed during differentiation in culture. Lev...Continue Reading

Citations

Aug 19, 2004·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Li ZouJianwen Zhu
Apr 25, 2000·Obstetrics and Gynecology·A SteinbornM Kaufmann
Jun 1, 2002·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Jacek R WilczyńskiJan Wilczyński
Sep 22, 2005·Immunology and Cell Biology·Alexander W PfaffErmanno Candolfi
Sep 1, 1996·American Journal of Reproductive Immunology : AJRI·M D SrivastavaB I Srivastava
Jul 7, 1999·American Journal of Reproductive Immunology : AJRI·Y DanielJ B Lessing
Dec 22, 2007·American Journal of Nephrology·Sofia BouchlariotouIoannis Stefanidis
Jan 5, 2006·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·R MadazliZ Calay
Dec 3, 2014·Placenta·E S A EgalA M Altemani
Oct 4, 2011·International Journal of Surgery·Allison H MaciverA M James Shapiro
Oct 16, 2004·Journal of Korean Medical Science·Shin-Young KimEun Hee Cho
Jun 24, 2020·Nutrients·Andrea BalduitRoberta Bulla
Jul 12, 2018·Journal of Perinatal Medicine·Engin KorkmazerÖzgür Akkurt
Nov 5, 2020·Reproductive Sciences·Enoch Appiah Adu-GyamfiYing-Xiong Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.