PMID: 9536946Apr 16, 1998Paper

Expression of interleukin 1 beta and interleukin 1 beta converting enzyme by intestinal macrophages in health and inflammatory bowel disease

Gut
M E McAlindonY R Mahida

Abstract

In the lipopolysaccharide (LPS) stimulated peripheral blood monocyte, the precursor form of interleukin 1 beta (IL-1 beta, 31 kD) is processed by IL-1 beta converting enzyme (ICE) to the mature, bioactive form (17 kD). IL-1 beta is a proinflammatory cytokine which is likely to have a role in the pathogenesis of inflammatory bowel disease (IBD). To investigate the expression and processing of IL-1 beta and ICE by tissue macrophages from normal and IBD colonic mucosa. Mucosal biopsy specimens and lamina propria cells from normal and IBD colons were studied by reverse transcription polymerase chain reaction (RT-PCR), western blot analysis, and ELISA (enzyme linked immunosorbent assay). Normal colonic macrophages synthesised only the precursor form of IL-1 beta whereas in IBD the mature form was also produced. Similarly, cells from normal colonic mucosa synthesised ICE as the precursor (p45) only, whereas macrophages from IBD colons produced active (p20) ICE. Ac-Tyr-Val-Ala-Asp-CHO, a specific peptide aldehyde inhibitor of ICE, significantly reduced the amount of mature IL-1 beta released by isolated IBD macrophages (from a median of 1.2 (range 0.78-4.42) ng/ml to 0.43 (0.21-1.6) ng/ml; p < 0.01). Exposure of normal colonic macroph...Continue Reading

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Citations

Dec 24, 2010·Diseases of the Colon and Rectum·Liraz Avram-HananelBetty Schwartz
Nov 10, 2007·Clinical Reviews in Allergy & Immunology·Heidi GoodarziRichard L Gallo
Mar 13, 2013·Trends in Immunology·Ehud Zigmond, Steffen Jung
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Oct 7, 2006·Current Opinion in Gastroenterology·L Y Kam, S R Targan
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Jul 31, 2008·World Journal of Gastroenterology : WJG·Fausto Sanchez-MunozJesus-K Yamamoto-Furusho
Nov 10, 2012·World Journal of Gastroenterology : WJG·Györgyi MűzesFerenc Sipos
Dec 21, 2013·Inflammatory Bowel Diseases·Mairi H McLeanScott K Durum
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