PMID: 9432015Jan 31, 1998Paper

Expression of interleukin-5 by human bone marrow microvascular endothelial cells: implications for the regulation of eosinophilopoiesis in vivo

British Journal of Haematology
R MöhleS Rafii

Abstract

We have shown that bone marrow microvascular endothelial cells (BMEC) support growth and differentiation of haemopoietic progenitors in vitro by elaboration of haemopoietic cytokines. Since generation of eosinophils can be observed in these coculture experiments, and BMEC do not produce interleukin (IL)-3, we evaluated BMEC for expression of IL-5, a specific growth factor for the eosinophilic lineage. Using RT-PCR, IL-5 mRNA was expressed by BMEC after stimulation (12 h) with lipopolysaccharide (LPS), IL-1, IL-2 and phorbol myristate acetate (PMA), but not by resting BMEC, after stimulation with TNF-alpha or interferon (IFN)-gamma. Moreover, IFN-gamma suppressed expression of IL-5 in response to LPS and IL-2. The identity of the PCR products was confirmed by restriction enzyme digestion, which resulted in fragments of the predicted size. T lymphocytes were not present in the endothelial cultures as demonstrated by absence of CD2 mRNA. Using a sensitive (1 pg/ml) ELISA assay. IL-5 was detected after 48 h incubation of BMEC with IL-2 (4.1 pg/10(6) cells) or with a combination of LPS and IL-2 (4.8 pg). However, the number of eosinophils generated after 4 weeks coculture of CD34+ haemopoietic cells with BMEC was not increased by ad...Continue Reading

Citations

May 5, 1999·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·T LalaniA R Ahmed
May 5, 1999·Current Opinion in Hematology·G J Roboz, S Rafii
Nov 13, 2001·American Journal of Respiratory and Critical Care Medicine·R G StirlingK F Chung
Oct 6, 2009·International Immunopharmacology·Nadia S MarianoEdson Antunes
Aug 19, 2007·The Journal of Allergy and Clinical Immunology·Ilaria CecioniFabio Almerigogna
Jul 23, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G M GauvreauJ A Denburg
Apr 18, 2009·British Journal of Pharmacology·Z ZídekE Kmonícková
Jan 27, 2010·British Journal of Pharmacology·M C CalixtoE Antunes
Jul 28, 2004·Biochemical Pharmacology·Heloisa Helena de Araujo FerreiraGilberto De Nucci
Aug 10, 2000·The Journal of Allergy and Clinical Immunology·M B HoganK S Landreth
Mar 18, 2015·Biological Chemistry·Hiroyuki NojimaAlex B Lentsch

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