PMID: 11318979Apr 25, 2001Paper

Expression of isoforms and splice variants of the latent transforming growth factor beta binding protein (LTBP) in cultured human liver myofibroblasts

Liver
K Mangasser-StephanA M Gressner

Abstract

The activation of hepatic stellate cells (HSC) to extracellular matrix (ECM) producing myofibroblasts (MFB) is the key pathogenetic event in human liver fibrogenesis. Latent transforming growth factor beta binding protein (LTBP), a component of the profibrogenic large latent transforming growth factor (TGF)-beta complex, is suggested to be important for secretion, latency, storage and activation of TGF-beta in the ECM. This study was performed to identify the expression profile of all hitherto known LTBP isoforms and LTBP splice variants in conjunction with that of TGF-beta isoforms in cultured human liver MFB. Cultured human MFB were analyzed for TGF-beta and LTBP using reverse-transcription polymerase chain reaction (RT-PCR), sequence analysis, immunofluorescence staining, metabolic labeling, immunoprecipitation, and enzyme-linked immunosorbent assay (ELISA). Transcripts of all three TGF-beta isoforms, of all four LTBP isoforms and of nearly all splice variants of LTBP-1 and LTBP-4 so far known were detected. Metabolic labeling followed by immunoprecipitation with anti-LTBP-1 antibody revealed the synthesis of LTBP proteins. Secretion of free LTBP and LTBP integrated into the large latent TGF-beta complex was demonstrated by ...Continue Reading

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Citations

Oct 29, 2003·Current Opinion in Biotechnology·Boris Hinz, Giulio Gabbiani
Jun 21, 2013·Fibrogenesis & Tissue Repair·Ariel Bing-Shi TanMichael Raghunath
Jul 1, 2005·Breast Cancer Research : BCR·Michael SamoszukGuillaume Chorn
Oct 12, 2013·PloS One·Ralf WeiskirchenCarsten Bergmann
Aug 17, 2004·Critical Reviews in Clinical Laboratory Sciences·Marko HyytiäinenJorma Keski-Oja
Feb 15, 2007·The Journal of Investigative Dermatology·Boris Hinz
Dec 15, 2007·Journal of Hepatology·Hermann E WasmuthFrank Lammert
Sep 22, 2012·The Journal of Pathology·Franco KlingbergEric S White
Nov 3, 2010·The American Journal of Pathology·Bradley P SullivanJames P Luyendyk
Nov 9, 2016·Frontiers in Physiology·Jiří Kanta
Dec 7, 2018·International Journal of Molecular Sciences·Stefania CannitoMassimiliano Cadamuro
Jun 18, 2019·Journal of Cellular Physiology·Xia YiWeidong Li
Sep 13, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sofia TorresJ Ignacio Casal

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