Expression of keratin 1, keratin 10, desmoglein 1 and desmocollin 1 in the epidermis: possible downregulation by interleukin-4 and interleukin-13 in atopic dermatitis

European Journal of Dermatology : EJD
Ami TotsukaYuichiro Tsunemi

Abstract

In the pathogenesis of atopic dermatitis (AD), skin barrier dysfunction and T-helper (Th) type 2 immune reactions play important roles. Alterations in the stratum spinosum of AD have not been studied in much detail. In this study, we investigated the changes of structural proteins and adhesion molecules residing in the stratum spinosum of AD lesional skin, and whether Th2 cytokines including interleukin (IL)-4 and IL-13 alter the expression of these proteins. Skin samples were collected from patients with AD and from healthy controls. Normal human epidermal keratinocytes were cultured and differentiated in the presence or absence of either IL-4 or IL-13 in vitro. The expression of keratin 1, keratin 10, desmoglein 1 and desmocollin 1 was examined by immunofluorescence and western blotting. The expression of these proteins was downregulated in AD lesional skin, and IL-4 and IL-13 were shown to suppress their expression. These results suggest that the stratum spinosum is impaired in AD lesional skin, possibly by Th2 cytokines, which may be involved in the pathogenesis of AD.

Citations

Feb 2, 2018·Bioscience of Microbiota, Food and Health·Hiroshi AshigaiTakaaki Yanai
Feb 19, 2019·The Journal of Clinical Investigation·Elena GolevaDonald Ym Leung
Jan 24, 2021·The Journal of Investigative Dermatology·Taras LyubchenkoElena Goleva
Dec 19, 2020·The Journal of Allergy and Clinical Immunology·Anna Sophie KlaeschenThomas Bieber
Mar 4, 2021·International Journal of Molecular Sciences·Yeon Kyung NamWoong Mo Yang
Dec 29, 2020·Proceedings of the National Academy of Sciences of the United States of America·Aisling M TowellJoan A Geoghegan
Jul 13, 2021·Experimental Dermatology·Angelique N VoorbergMarie L A Schuttelaar
Sep 14, 2021·Toxicological Sciences : an Official Journal of the Society of Toxicology·Rachel BaurStacey E Anderson

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Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.