Expression of key genes affecting artemisinin content in five Artemisia species

Scientific Reports
Maryam SalehiSajad Rashidi Monfared

Abstract

Artemisinin, an effective anti-malarial drug is synthesized in the specialized 10-celled biseriate glandular trichomes of some Artemisia species. In order to have an insight into artemisinin biosynthesis in species other than A. annua, five species with different artemisinin contents were investigated for the expression of key genes that influence artemisinin content. The least relative expression of the examined terpene synthase genes accompanied with very low glandular trichome density (4 No. mm-2) and absence of artemisinin content in A. khorassanica (S2) underscored the vast metabolic capacity of glandular trichomes. A. deserti (S4) with artemisinin content of 5.13 mg g-1 DW had a very high expression of Aa-ALDH1 and Aa-CYP71AV1 and low expression of Aa-DBR2. It is possible to develop plants with high artemisinin synthesis ability by downregulating Aa-ORA in S4, which may result in the reduction of Aa-ALDH1 and Aa-CYP71AV1 genes expression and effectively change the metabolic flux to favor more of artemisinin production than artemisinic acid. Based on the results, the Aa-ABCG6 transporter may be involved in trichome development. S4 had high transcript levels and larger glandular trichomes (3.46 fold) than A. annua found in ...Continue Reading

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Citations

Jan 28, 2020·Trends in Plant Science·Camille ChalvinAdnane Boualem

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Datasets Mentioned

BETA
AJ001539

Methods Mentioned

BETA
fluorescence microscopy
transgenic
flow cytometry
Fluorescence
PCR
electrophoresis

Software Mentioned

Oligo 7
FloMax
Oligoanalyzer
SAS
FACSDiva
BD

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