Expression of KLF6-SV2 in colorectal cancer and its impact on proliferation and apoptosis

European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)
Biao ZhangYan-An Wu

Abstract

Kruppel like factor 6 (KLF6), a member of KLF family, which has classic zinc finger structure, is broadly considered to have anticancer activity. The role of SV2 variant, one of KLF6 alternative splicing isoforms has not yet been definite in the colorectal cancer. This study aimed to detect the expression of the KLF6-SV2 in colorectal cancer and investigate its impact on cell proliferation and apoptosis. qRT-PCR was used to quantitatively determine KLF6-SV2 mRNA expression in colorectal cancer samples, corresponding normal tissue, normal colonic mucosal cell line FHC and seven colorectal cancer cell lines. SW480 and SW620 cell models with over-expressing KLF6-SV2 were constructed. Cell proliferation, cell cycle and apoptosis were measured respectively using MTT assay, DNA ploidy detection and Annexin V flow cytometry. Meanwhile, expression of p53, p21 and Bax were detected by qRT-PCR and western blot. The mRNA expression level of KLF6-SV2 in colorectal cancer tissues (0.783±0.409) was decreased than in corresponding normal tissues (1.086±0.449) (P<0.01), and expression in SW480 and SW620 were lower than in FHC, HCT116, LoVo, HT29, Caco-2 and RKO. In cell lines over-expressing KLF6-SV2, cell proliferation was markedly suppressed...Continue Reading

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Citations

Nov 30, 2018·Cell Proliferation·Rong WangYongqian Shu
Oct 2, 2020·Biomolecules·Saiful E SyafruddinTeck Yew Low
Jun 2, 2021·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·X LeónM Camacho

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Methods Mentioned

BETA
PCR
electrophoresis
flow cytometry
transfection

Software Mentioned

SPSS

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