Expression of Kunitz protease inhibitor--containing forms of amyloid beta-protein precursor within vascular thrombi

Circulation
I M LangR R Schleef

Abstract

The presence of patent neovessels within vascular occlusions in chronic thromboembolic pulmonary hypertension suggests that local mechanisms exist to regulate the coagulation system. This study investigated the expression of a potent inhibitor of Factor IXa and Factor XIa (ie, protease nexin-2/ amyloid beta-protein precursor, A beta PP) in the organized vascular occlusions harvested from patients with this disease. Immunohistochemical analysis revealed intense immunoreactivity for A beta PP in the single layer of cells that line the neovessels. A positive signal was also detected by in situ hybridization analysis with the use of a 35S-UTP-labeled antisense riboprobe that recognizes the various alternatively spliced mRNA forms of this molecule. To identify the forms of A beta PP produced within the thrombi, total RNA was extracted from the thrombi, reverse transcribed, and subjected to amplification with the use of the polymerase chain reaction (PCR) and primers that flank the region encoding the alternatively spliced 56-amino acid Kunitz-type protease inhibitor (KPI) domain. The major PCR products consisted of 255 bp and 312 bp and corresponded to transcripts encoding this domain (ie, A beta PP751 and A beta PP770). In situ hyb...Continue Reading

References

Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J D BuxbaumP Greengard
Sep 1, 1992·Journal of Neuroscience Research·B WolozinT Sunderland
Dec 26, 1992·Annals of the New York Academy of Sciences·H M Wisniewski, J Wegiel
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A TamaokaD J Selkoe
Feb 1, 1992·Neuropathology and Applied Neurobiology·F CoriaJ Ghiso
Jan 1, 1992·Journal of Molecular Neuroscience : MN·C A MarottaB Tate
Jan 1, 1991·Annals of the New York Academy of Sciences·W E Van NostrandD D Cunningham
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D GoldgaberD C Gajdusek
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B Tate-OstroffC A Marotta
Jan 1, 1988·Alzheimer Disease and Associated Disorders·D E SchmechelA D Roses
Mar 1, 1988·Annals of Internal Medicine·S RichB H Brundage
Dec 1, 1983·Chest·J R BenottiJ E Dalen
Oct 1, 1994·Current Opinion in Neurobiology·D J Selkoe
Apr 15, 1994·Journal of Neuroscience Research·J R CiallellaJ P McGillis
Nov 1, 1993·The Journal of Clinical Investigation·A H SchmaierW E Van Nostrand
Jun 1, 1993·Brain Research. Molecular Brain Research·P BucklandP McGuffin

❮ Previous
Next ❯

Citations

Aug 30, 2016·Annals of the American Thoracic Society·Irene M LangAnton Vonk Noordegraaf
Jun 3, 2016·Pulmonary Circulation·Daniel T Matthews, Anna R Hemnes
Dec 9, 2016·Physiology·Livius V d'UscioZvonimir S Katusic
Sep 30, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Livius V d'UscioZvonimir S Katusic
Aug 2, 2017·Journal of Thrombosis and Haemostasis : JTH·M-P WinterI M Lang
Feb 6, 2021·Clinics in Chest Medicine·Irene M LangNika Skoro-Sajer
Aug 24, 2021·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Marc de PerrotWilliam R Auger

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Cardiology Journals

Discover the latest cardiology research in this collection of the top cardiology journals.

Related Papers

Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis
Jerzy WegielA Giovanni
Annals of the New York Academy of Sciences
H M WisniewskiJ Frackowiak
Journal of the Neurological Sciences
Barbara BorroniAlessandro Padovani
© 2021 Meta ULC. All rights reserved