Expression of Lactococcus lactis NADH oxidase increases 2,3-butanediol production in Pdc-deficient Saccharomyces cerevisiae

Bioresource Technology
Jin-Woo KimJin-Ho Seo

Abstract

To minimize glycerol production during 2,3-BD fermentation by the engineered Saccharomyces cerevisiae, the Lactococcus lactis water-forming NADH oxidase gene (noxE) was expressed at five different levels. The expression of NADH oxidase substantially decreased the intracellular NADH/NAD(+) ratio. The S. cerevisiae BD5_T2nox strain expressing noxE produced 2,3-BD with yield of 0.359 g 2,3-BD/gglucose and glycerol with 0.069gglycerol/gglucose, which are 23.8% higher and 65.3% lower than those of the isogenic strain without noxE. These results demonstrate that the carbon flux could be redirected from glycerol to 2,3-BD through alteration of the NADH/NAD(+) ratio by the expression of NADH oxidase.

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Citations

Apr 21, 2017·Critical Reviews in Biotechnology·Taowei YangShang-Tian Yang
Jun 7, 2017·FEMS Yeast Research·Quinten DeparisJohan M Thevelein
Mar 28, 2018·Journal of Industrial Microbiology & Biotechnology·Jiaheng LiuJianjun Qiao
Feb 17, 2017·Applied Microbiology and Biotechnology·Soo-Jung KimJin-Ho Seo
Oct 9, 2020·Current Opinion in Chemical Biology·Francesca V GambacortaBrian F Pfleger
Jul 22, 2021·Applied Microbiology and Biotechnology·Jae Won LeeChristopher V Rao
Sep 16, 2021·Applied and Environmental Microbiology·Sadat Mohamed Rezk Khattab, Takashi Watanabe

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