Expression of laminin and type IV collagen by basement membrane-producing EHS tumors in streptozotocin-induced diabetic mice: in vivo modulation by low-molecular-weight heparin fragments

Biochemical Pharmacology
C Asselot-ChapelP Kern

Abstract

The biosynthesis of basement membrane components in Engelberth Holm Swarm-bearing mice with or without streptozotocin-induced diabetes and the effect of low-molecular-weight heparin derivatives (CY222, Sanofi Recherche/Institut Choay) on the relative rates of these synthetic activities were studied. In diabetic mice, the laminin mRNA level increased, whereas type IV collagen mRNA decreased. In vivo treatment with heparin fragments decreased the mRNA level of laminin to control values without altering the mRNA level of collagen IV. Biosynthetic studies with radiolabeled precursors ([3H]-proline for collagen and [35S]-methionine for laminin) confirmed these results. Laminin protein biosynthesis increased in diabetic mice. Treatment with CY222 corrected this alteration. Our results suggested an increased labeling of polymeric forms of collagen IV in diabetic mice. In addition, we showed that biosynthesis of acid-extractable collagen IV decreased in diabetic mice and that CY222 treatment corrected this disturbance. These experiments suggest that low-molecular-weight heparin fragments CY222 can modulate the biosynthesis of extracellular matrix macromolecules altered in diabetic animals by different pathways, including pretranslation...Continue Reading

References

Aug 15, 1979·The Biochemical Journal·P KernL Robert
Mar 1, 1978·European Journal of Biochemistry·R TimplH Wiedemann
Jan 1, 1977·The Journal of Experimental Medicine·R W OrkinR Swarm
Jul 1, 1990·Experimental Eye Research·R PoulsomR P Boot-Handford
Apr 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P D Yurchenco, J C Schittny
Apr 1, 1989·European Journal of Biochemistry·R Timpl
Oct 1, 1989·Diabetes·R L Engerman
Jun 1, 1986·The Biochemical Journal·J T GallagherW P Steward
Aug 1, 1986·The Journal of Pathology·D R Abrahamson
May 19, 1988·The New England Journal of Medicine·M BrownleeH Vlassara
Aug 1, 1988·The Journal of Clinical Investigation·E CaglieroM Lorenzi
Aug 5, 1987·The Journal of Biological Chemistry·T PikkarainenK Tryggvason
Aug 15, 1984·European Journal of Biochemistry·S FujiwaraJ Engel
Dec 15, 1983·European Journal of Biochemistry·R TimplG Wick
Jan 1, 1994·Methods in Enzymology·P D Yurchenco, J J O'Rear
Dec 1, 1994·The Journal of Surgical Research·A SankT L Tuan
Jan 1, 1994·The Journal of Clinical Investigation·S RoyM Lorenzi
Apr 1, 1996·Journal of Biomedical Materials Research·D LogeartP Kern

❮ Previous
Next ❯

Citations

Jun 16, 2005·Biochimica Et Biophysica Acta·Cilene R de LimaYara M Michelacci

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.

Related Papers

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
Tanya M OsickaWayne D Comper
Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
L R FerreiraJ J Sauk
© 2022 Meta ULC. All rights reserved