Expression of lauroyl-acyl carrier protein thioesterase in brassica napus seeds induces pathways for both fatty acid oxidation and biosynthesis and implies a set point for triacylglycerol accumulation

The Plant Cell
V S Eccleston, J Ohlrogge

Abstract

Expression of a California bay lauroyl-acyl carrier protein thioesterase (MCTE) in developing seeds of transgenic oilseed rape alters the fatty acid composition of the mature seed, resulting in up to 60 mol% of laurate in triacylglycerols. In this study, we examined the metabolism of lauric acid and 14C-acetate in developing seeds of oilseed rape that express high levels of MCTE. Lauroyl-CoA oxidase activity but not palmitoyl-CoA oxidase activity was increased several-fold in developing seeds expressing MCTE. In addition, isocitrate lyase and malate synthase activities were six- and 30-fold higher, respectively, in high-laurate developing seeds. Control seeds incorporated 14C-acetate almost entirely into fatty acids, whereas in seeds expressing MCTE, only 50% of the label was recovered in lipids and the remainder was in a range of water-soluble components, including sucrose and malate. Together, these results indicate that the pathways for beta-oxidation and the glyoxylate cycle have been induced in seeds expressing high levels of MCTE. Although a substantial portion of the fatty acid produced in these seeds is recycled to acetyl-CoA and sucrose through the beta-oxidation and glyoxylate cycle pathways, total seed oil is not red...Continue Reading

Citations

Apr 21, 1999·Current Opinion in Biotechnology·D J Murphy
Feb 15, 2001·Trends in Plant Science·P J Eastmond, I A Graham
Oct 26, 1999·Trends in Plant Science·M J Hills
Apr 2, 2003·Current Opinion in Plant Biology·Jan Jaworski, Edgar B Cahoon
Sep 29, 1998·The Plant Journal : for Cell and Molecular Biology·K DeheshJ Byrne
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·M A HooksI A Graham
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·V MittendorfY Poirier
Oct 9, 2002·The Plant Journal : for Cell and Molecular Biology·Martin FuldaErnst Heinz
Jun 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Carl AndreJohn Shanklin
May 1, 2008·Annual Review of Plant Biology·Ian A Graham
Nov 4, 2011·Microbial Cell Factories·Wei-Luen YuMichael D Burkart
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·E LogemannK Hahlbrock
Jan 9, 2014·Proceedings of the National Academy of Sciences of the United States of America·Philip D BatesJohn Browse
Mar 9, 2000·Trends in Plant Science·A A MillarL Kunst
Feb 2, 2010·Journal of Plant Physiology·A J Moreno-PérezJ J Salas
Apr 5, 2008·Progress in Lipid Research·Robert E Minto, Brenda J Blacklock
Jun 6, 2009·Plant Biotechnology Journal·Loreta Gudynaite-SavitchBrian L A Miki
Mar 2, 2006·The Plant Journal : for Cell and Molecular Biology·Elizabeth L RylottIan A Graham
Nov 22, 2011·European Journal of Lipid Science and Technology : EJLST·Anders S CarlssonPer Hofvander
Nov 30, 2012·Plant Biotechnology Journal·Thomas VanherckeAllan G Green
Apr 2, 2008·Current Opinion in Biotechnology·Jörg Schwender
Jun 12, 2004·Plant Physiology and Biochemistry : PPB·Joaquín J SalasRafael Garcés
Nov 18, 2005·Progress in Lipid Research·Florence BouvierBilal Camara
Nov 25, 2014·Plant Biotechnology Journal·Meghna R MalikKristi D Snell
Jul 16, 2013·Plant Science : an International Journal of Experimental Plant Biology·Sarah K Kessel-VigeliusNicole Linka
Jan 22, 2002·Metabolic Engineering·Jay J Thelen, John B Ohlrogge
Feb 5, 2016·Annual Review of Plant Biology·Changcheng Xu, John Shanklin

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.