PMID: 18195492Jan 16, 2008Paper

Expression of leptin and long form of leptin receptor genes and proteins in pituitary of cyclic and pregnant pigs

Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society
G SiawrysJ Przala

Abstract

Leptin is a multifunctional regulator in numerous tissues, including the pituitary. It is not known, whether the porcine pituitary is a source of leptin synthesis and possesses the leptin receptor protein. It is also unknown, if a relationship exists between expression levels of these proteins in the pituitary and physiological status of sows. Therefore, the aim of the study was 1] to examine, by Western-blotting analysis, the expression levels of leptin and the long form of leptin receptor (OB-Rb) in the porcine anterior (AP) and posterior (NP) pituitary gland during mid- and late-luteal phases of the oestrous cycle (days 10 - 12 and 14 - 16) as well as during two stages of early pregnancy (days 14 - 16 and 30 - 32); and 2] to localise, using in situ hybridisation method (ISH), the expression of leptin and OB-Rb genes in the pituitary gland in the above mentioned stages of the cycle and pregnancy. Western-blotting analysis showed that leptin protein expression in AP was higher in the late-luteal phase than in the mid-luteal phase, while OB-Rb protein expression in both lobes was higher in the mid-luteal phase. In turn, during pregnancy leptin protein content in AP and OB-Rb protein content in NP were more pronounced on days 14...Continue Reading

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