Expression of lipases and lipid receptors in sperm storage tubules and possible role of fatty acids in sperm survival in the hen oviduct

Theriogenology
A HuangYukinori Yoshimura

Abstract

The aim of this study was to determine the role of fatty acids for sperm survival in the sperm storage tubules (SSTs) of the hen oviduct. The mucosa tissues of uterovaginal junction (UVJ) of White Leghorn laying hens with or without artificial insemination using semen from Barred Plymouth Rock roosters were collected. The lipid density in the epithelium of UVJ and SST was analyzed by Sudan black B staining. The expressions of genes encoding lipid receptors and lipases were assayed by polymerase chain reaction in UVJ mucosa and SST cells isolated by laser microdissection. Fatty acid composition was analyzed by gas chromatography, and sperm were cultured with or without the identified predominant fatty acids for 24 hours to examine their effect on sperm viability. The lipid droplets were localized in the epithelium of UVJ mucosa and SSTs. The expression of genes encoding very low-density lipoprotein receptor(VLDLR), low-density lipoprotein receptor (LDLR), and fatty acid translocase (FAT/CD36) were found in SST cells. Expression of genes encoding endothelial lipase (EL), lipase H (LIPH), adipose triglyceride lipase (ATGL), and lipoprotein lipase (LPL) were found in UVJ. In contrast, only ATGL was found in SST cells, and its expre...Continue Reading

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Citations

Oct 12, 2018·The Journal of Reproduction and Development·Hsiu-Lin HuangYu-Shin Cheng
Nov 24, 2019·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Asrat Tera DoleboJoram M Mwacharo
Apr 9, 2020·Journal of Animal Science and Biotechnology·Chaoliang WenNing Yang
Jan 20, 2017·BMC Genomics·Mohammad AtikuzzamanHeriberto Rodriguez-Martinez
Jan 30, 2019·International Journal of Molecular Sciences·Manuel Alvarez-RodriguezHeriberto Rodriguez-Martinez
Aug 6, 2021·Royal Society Open Science·Katherine AssersohnNicola Hemmings

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