Expression of lymphokine genes in splenic lymphocytes of autoimmune mice

Molecular Immunology
S UmlandC Martens

Abstract

Lymphocytes of autoimmune mice have been reported to have defective IL-2 production and proliferation in response to the mitogen concanavalin A. We have examined transcription of lymphokine genes in Con A stimulated spleen cells from both autoimmune and normal mice and found that IL-2, IL-4 and gamma-interferon (IFN gamma) were induced in all mice tested. Spleen cells were taken from young (pre-disease) or old (clinically active) MRL/lpr (lpr) and male BXSB autoimmune mice and from their normal counterparts (MRL/n, BXSB females, BALB/c and DBA/2) and stimulated with Con A. Con A induced production of IL-2, IL-4 and IFN gamma message and protein, and kinetics of induction did not vary significantly among the strains. However, in old lpr mice, levels of IL-2 protein and mRNA were about 10-fold lower than in other strains; IL-4 protein and mRNA were decreased approximately three-fold; and IFN gamma mRNA was readily detected in unstimulated cells and low but detectable levels of protein were produced constitutively. In contrast, little or no defect in IL-2 or IL-4 transcription or secretion were seen in male BXSB mice and no constitutive IFN gamma transcription was seen in this strain. These data indicate that all three lymphokine ...Continue Reading

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Citations

Nov 1, 1995·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·H KamadaH Nagai
Apr 1, 1995·Immunological Reviews·D M Klinman, A D Steinberg
Aug 1, 1990·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·R Jonsson, R Holmdahl
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May 15, 1991·Biochemical and Biophysical Research Communications·L Mengle-GawD C Tiemeier

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