Expression of major histocompatibility complex class II molecules in HeLa cells promotes the recruitment of AP-1 Golgi-specific assembly proteins on Golgi membranes.

The Journal of Biological Chemistry
J SalameroB Hoflack

Abstract

The newly synthesized major histocompatibility complex (MHC) class II molecules, an alphabeta dimer associated with the Ii invariant chain, must be targeted to endosomal, lysosomal enzyme-rich compartments in order to bind and present immunogenic peptides. The precise route followed by this complex at the exit of the trans-Golgi network, the last sorting station of the biosynthetic pathway, is poorly understood. We show here that overexpression of alphabetaIi complexes in HeLa cells promotes the first step of clathrin-coat assembly in vitro, that is the ARF-dependent translocation of AP-1 Golgi-specific assembly proteins on membranes. In contrast, alphabeta dimers alone or associated with Ii lacking most of its cytoplasmic domain fail to recruit AP-1. This study strongly suggests that the invariant chain (Ii) is responsible for the AP-1-dependent sorting of the alphabeta dimers in the trans-Golgi network of HeLa cells and that the MHC class II molecules are, like the mannose 6-phosphate receptors, transported directly from this compartment to endosomes via clathrin-coated vesicles.

References

Mar 1, 1992·The Journal of Cell Biology·R D KlausnerJ Lippincott-Schwartz
Aug 1, 1991·Current Opinion in Cell Biology·J J NeefjesH L Ploegh
Jan 1, 1989·Annual Review of Cell Biology·S Kornfeld, I Mellman
Aug 1, 1995·Current Opinion in Cell Biology·I MellmanS Amigorena
Aug 1, 1994·Current Opinion in Cell Biology·M S Robinson
Jan 1, 1994·Annual Review of Immunology·P Cresswell
Nov 1, 1993·The Journal of Cell Biology·L M TraubS Kornfeld
Sep 1, 1993·Medical Microbiology and Immunology·S BhakdiM Palmer
Aug 1, 1993·Current Opinion in Cell Biology·C V Harding, H J Geuze
Oct 1, 1993·The Journal of Cell Biology·J N Glickman, S Kornfeld
Mar 1, 1996·The Journal of Cell Biology·J N GlickmanH J Geuze

❮ Previous
Next ❯

Citations

Feb 28, 1998·Immunologic Research·M S Marks
Aug 1, 1997·Current Opinion in Cell Biology·T KirchhausenH Riezman
Aug 1, 1997·Current Opinion in Cell Biology·L M Traub, S Kornfeld
Feb 11, 2003·Cell Structure and Function·Graça RaposoMichael S Marks
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·P Stumptner-CuveletteP Benaroch
Apr 21, 1997·The Journal of Cell Biology·R Le Borgne, B Hoflack
Mar 7, 2001·The Journal of Investigative Dermatology·J SalameroD Hanau
Jun 21, 2016·Immunological Reviews·Peter van Endert
Aug 19, 2000·The Journal of Biological Chemistry·C AngenieuxH de La Salle
Jan 4, 2003·Nature Reviews. Immunology·D Branch Moody, Steven A Porcelli
Mar 18, 2000·The Journal of Biological Chemistry·D G RodionovO Bakke
Feb 6, 1999·The Journal of Biological Chemistry·M AridorW E Balch
Feb 20, 1999·The Journal of Biological Chemistry·D ZizioliP Schu
Aug 31, 2000·The Journal of Biological Chemistry·A BrunetJ Thibodeau
Aug 8, 1997·The Journal of Biological Chemistry·S HöningK von Figura
Sep 17, 2002·Traffic·Graça RaposoHans Geuze
Dec 25, 2012·Journal of Cell Science·Marie-Claude Bourgeois-Daigneault, Jacques Thibodeau
Feb 21, 2002·Biochimica Et Biophysica Acta·Pamela Stumptner-Cuvelette, Philippe Benaroch
Aug 26, 1998·Biochimica Et Biophysica Acta·R Le Borgne, B Hoflack

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.