Expression of Mammalian Target of Rapamycin and Downstream Targets in Normal and Gestational Diabetic Human Term Placenta

Reproductive Sciences
Leyla SatiCiler Celik-Ozenci

Abstract

Mammalian target of rapamycin (mTOR) signaling serves as a central regulator of cell growth, proliferation, and survival by interacting with various proteins. To date, few studies implicated mTOR in placenta. Human placenta in gestational diabetes mellitus (GDM) shows several alterations including villous immaturity, impaired placental function, and overgrowth. Hence, we aimed to investigate the expression of mTOR, phospho-mTOR (p-mTOR), and the 2 phosphorylated downstream targets of mTOR, ribosomal protein S6 kinase 1 (p-p70S6K), and eukaryotic initiation factor 4E-binding protein 1 (p-4EBP1) in normal term and gestational diabetic human placentas. Immunohistochemistry and Western blot were performed with antibodies against mTOR, p-mTOR, p-p70S6K, and p-4EBP1 (Thr37/46) in normal and diabetic placentas (n = 6 each) and quantified by ImageJ. All mTOR pathway components that we studied were immunolocalized in both normal and diabetic placenta groups. Syncytiotrophoblast and the vascular wall in villi displayed cytoplasmic mTOR and p-mTOR (S2448) immunoreactivities in all placenta samples. However, increased expression of p70S6K in syncytiotrophoblast and p-4EBP1 (Thr37/46) in villous stromal cells was observed in gestational dia...Continue Reading

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Citations

Jun 26, 2018·Molecular Nutrition & Food Research·Evangelina CapobiancoAlicia Jawerbaum
Aug 16, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ramkumar MohanEmilyn Uy Alejandro
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May 14, 2020·Biology of Reproduction·Jie DongXiaohong Wang
Nov 7, 2017·Journal of Diabetes Research·Roberto Villalobos-LabraLuis Sobrevia
Dec 4, 2020·Physiological Reviews·Daniel J HoffmanDaniel B Hardy
Dec 17, 2020·Frontiers in Cell and Developmental Biology·Fredrick J RosarioThomas Jansson
Nov 27, 2020·Food & Nutrition Research·Yonghua ZhangBo Chen
Feb 16, 2021·American Journal of Physiology. Endocrinology and Metabolism·Hefei WangJian V Zhang
May 12, 2021·Obstetrics and Gynecology Clinics of North America·Jerad H DumoltThomas Jansson

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Methods Mentioned

BETA
Cesarean section
protein assay
Electrophoresis

Software Mentioned

Spot Imaging
ImageJ
Excel
SigmaStat

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