PMID: 22333609Feb 16, 2012Paper

Expression of mammalian target of rapamycin in colon cancer and its effect on proliferation and apoptosis of human colon cancer HT-29 cells

Zhonghua yi xue za zhi
Zhi-yong Zhang, Xie-fu Zhang

Abstract

To explore the expression of mammalian target of rapamycin (mTOR) in colon cancer and the inhibitory effect of mTOR siRNA on the proliferation and apoptosis of human colon cancer HT-29 cells. Immunohistochemistry was employed to detect the expression of phosphorylated mTOR (p-mTOR) in colon cancer tissues (n = 50) and normal colon tissues (n = 50) from First Affiliated Hospital of Zhengzhou University (October 2009 to June 2010). The correlations were examined between the expression of p-mTOR and such clinical pathological data as colon cancer staging and lymph node metastasis. The oligonucleotide templates of mTOR siRNA were designed and employed to transfect HT-29. The nonsense control groups were established accordingly by siRNA with an insignificant order. And the blank group had no transfection. The protein level of mTOR was measured by Western blotting. The method of MTT was used to detect the cell proliferation. And the method of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) was employed to detect the apoptosis. The expression of p-mTOR was higher in the colon cancer group than the control group [60% (30 cases) vs 14% (7 cases), P < 0.05]. And the expression level was correlated with the degree of ...Continue Reading

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis