PMID: 11919265Mar 29, 2002Paper

Expression of Manduca sexta V-ATPase genes mvB, mvG and mvd is regulated by ecdysteroids

The Journal of Experimental Biology
S ReinekeH Merzendorfer

Abstract

V-ATPases are complex proteins consisting of a peripheral, ATP-hydrolysing V(1) complex and a membrane-bound H(+)-translocating V(o) complex. The plasma membrane V-ATPase from the tobacco hornworm (Manduca sexta) midgut is made up of eight different V(1) and four different V(o) subunits. During starvation and moulting, V-ATPase activity decreases as a result of the dissociation of the V(1) complex from the V(o) complex. To determine whether subunit biosynthesis is reduced during periods of enzyme inactivity, we measured the transcript levels and transcriptional activities of V-ATPase genes. Northern blots revealed the downregulation of almost all V-ATPase transcripts during starvation. During moulting, transcript levels of the three V-ATPase genes examined, mvB, mvG and mvd, also decreased, and this decrease was negatively correlated with the titre of 20-hydroxyecdysone (20-HE) and positively correlated with the titre of juvenile hormone (JH). To test the biological significance of these correlations, we injected both hormones into feeding larvae and measured transcript levels several hours later. A short-term increase and a long-term decrease in levels of mRNA were observed after 20-HE injection, whereas JH injection had no si...Continue Reading

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