Expression of matrix metalloproteases (MMP-2, MT1 -MMP) and their tissue inhibitor (TIMP-2) by rat sertoli cells in culture: implications for spermatogenesis

Biological Chemistry
M Liliana SlongoMaurizio Onisto

Abstract

During testicular development and maturation, extracellular matrix (ECM) remodelling is a fundamental process which requires the presence of several proteases and protease inhibitors. Among the proteases, a pivotal role has been proposed for matrix metalloproteases (MMPs) and their tissue inhibitors (TIMPs). Here we report an analysis of MMP-2, MT1-MMP and TIMP-2 expression by rat Sertoli cells in culture using RT-PCR and zymographic techniques. Stimulating Sertoli cells with follicle-stimulating hormone (FSH) in vitro induced evident changes in the level of their mRNA in a time-dependent manner. In the case of TIMP-2 and MT1-MMP, the respective transcripts were augmented up to three-fold after 24 h of treatment, and MMP-2 transcripts increased by four times in the same period. MMP-2 activity determined by gelatin zymography showed an increase in enzyme secretion after FSH stimulation. The results of this study suggest that: (i) at the surface of Sertoli cells pro-MMP-2 activation mediated by MT1-MMP may occur, involving TIMP-2 as a receptor; and (ii) the expression of these molecules is not constitutive in this cell type, but may be modulated by FSH and is therefore implicated in spermatogenesis.

References

Dec 1, 1990·Biology of Reproduction·Q X SangS W Byers
May 1, 1994·Analytical Biochemistry·D E Kleiner, W G Stetler-Stevenson
Feb 18, 1999·Molecular and Cellular Endocrinology·S ScarpinoC Boitani
Sep 1, 1999·Clinical Endocrinology·E NieschlagG F Weinbauer
May 29, 2000·The Journal of Biological Chemistry·J J CaterinaH Birkedal-Hansen

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Citations

Mar 28, 2008·Archives of Oral Biology·Lokesh SuriEleni Gagari
May 26, 2010·International Journal of Andrology·U SilvánJ Aréchaga
Mar 1, 2013·Asian-Australasian Journal of Animal Sciences·Sang-Hwan KimJong-Taek Yoon
Jan 3, 2007·Prostate Cancer and Prostatic Diseases·Q WeiY Li
Dec 29, 2006·The Journal of Biological Chemistry·Pei-Li YaoJohn H Richburg
Jul 12, 2019·Scandinavian Journal of Clinical and Laboratory Investigation·Ewa MatuszczakAdam Hermanowicz
Jan 25, 2020·Theriogenology·Gabriel Ribas PereiraIvan Cunha Bustamante-Filho

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