Expression of messenger ribonucleic acid (mRNA) encoding 3beta-hydroxysteroid dehydrogenase delta4,delta5 isomerase (3beta-HSD) during recruitment and selection of bovine ovarian follicles: identification of dominant follicles by expression of 3beta-HSD mRNA within the granulosa cell layer

Biology of Reproduction
B BaoR S Youngquist

Abstract

The objective of the present study was to examine changes in expression of mRNA encoding 3beta-hydroxysteroid dehydrogenase delta4,delta5 isomerase (3beta-HSD) during recruitment and selection of bovine ovarian follicles. Dairy heifers (4-5/time period) were ovariectomized at 12, 24, 36, 48, 60, 72, 84, or 96 h after initiation of the first follicular wave (Time 0) following estrus. Expression of 3beta-HSD mRNA was localized by in situ hybridization and quantified by image analysis. Expression of 3beta-HSD mRNA was first detected in theca interna cells of preantral follicles with a well-developed theca layer and in granulosa cells of follicles > or = 8 mm in diameter. Regardless of stage of follicular wave, expression of 3beta-HSD mRNA in granulosa cells of follicles > or = 8 mm was correlated with follicular size (r = 0.665; p < 0.01). The 36-h time period appeared to be a transition period for selection since dominant follicles were detected by size and expression of 3beta-HSD mRNA in some cows but not in others. By 48 h after wave initiation, dominant follicles could be identified by both size and expression of 3beta-HSD mRNA. Expression of mRNA for 3beta-HSD in theca cells was higher (p < 0.05) at 24 h than at 12 h and rema...Continue Reading

Citations

May 26, 2004·Domestic Animal Endocrinology·J H HamptonH A Garverick
Jul 27, 2002·Domestic Animal Endocrinology·M Mihm, E J Austin
Aug 14, 2002·Reproduction in Domestic Animals = Zuchthygiene·M MihmE J Austin
Jun 4, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·J H HamptonH A Garverick
May 17, 2007·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Christian De GeyterMaria De Geyter
Aug 7, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Helen F Irving-RodgersRaymond J Rodgers
Aug 22, 2001·Biology of Reproduction·J E FortuneA M Turzillo
Oct 26, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H MalekinejadJ Fink-Gremmels

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