Expression of messenger ribonucleic acids of insulin-like growth factor binding protein-2, -4, and -5 in the ovine ovary: localization and changes during growth and atresia of antral follicles

Biology of Reproduction
N BesnardP Monget

Abstract

In the sheep as in many mammalian species, growth and atresia of antral follicles are characterized, respectively, by a decrease and a high increase in the intrafollicular levels of insulin-like growth factor binding proteins of less than 40 kDa (IGFBPs < 40 kDa), mainly IGFBP-2, -4, and -5. The objective of this study was to investigate whether such changes are associated with changes in follicular expression of the corresponding mRNA. For this purpose, ovaries were recovered from ewes slaughtered at the end of follicular phase (i.e., 30 h after progestagen sponge removal; control ewes) or at 24 h, 36 h or 72 h after hypophysectomy (hypox) performed 30 h after sponge removal. The expression of mRNA of IGFBPs of less than 40 kDa (IGFBPs < 40 kDa mRNA) was studied in ovine antral follicles from control and hypox ewes by in situ hybridization using [35S]-labeled human IGFBP-2, -4, and -5 cRNA as probes. In control ewes, IGFBP-2 mRNA was mainly expressed in granulosa as a gradient in healthy follicles, the expression being higher in granulosa cells close to the basal membrane than in granulosa cells bordering the antrum and within the cumulus. The level of IGFBP-2 mRNA was lower both in granulosa cells close to the basal membrane ...Continue Reading

Citations

May 23, 2013·Animal Reproduction Science·F M RodríguezF Rey
May 5, 2004·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Sabine MazerbourgPhilippe Monget
Aug 14, 2001·Molecular and Cellular Endocrinology·C S Chamberlain, L J Spicer
Aug 30, 2000·Molecular and Cellular Endocrinology·P Monget, C Bondy
Jul 27, 2002·Domestic Animal Endocrinology·Philippe MongetDanielle Monniaux
Mar 1, 2009·Animal : an International Journal of Animal Bioscience·G Tosser-KloppM Sancristobal
Mar 23, 2006·Reproductive Biology and Endocrinology : RB&E·Svetlana UzbekovaPascal Mermillod
May 6, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·A BonnetG Tosser-Klopp
Jun 5, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Jennifer L JuengelDouglas C Eckery
Mar 4, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Kirsty A WaltersEvelyn E Telfer
Aug 31, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·E D WatsonD G Armstrong
Oct 22, 2008·Molecular Reproduction and Development·Zhilin LiuEric Antoniou
Oct 12, 2004·Biochemical and Biophysical Research Communications·Sébastien DadéPhilippe Monget
Jul 17, 1998·Frontiers in Neuroendocrinology·N Chabbert BuffetP Bouchard
Feb 6, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Véronique CadoretPhilippe Monget
Aug 24, 1999·Endocrine Reviews·L PoretskyL C Giudice
Sep 25, 2019·BMC Research Notes·Ahmed A SalehYongju Zhao

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