Expression of methionine aminopeptidase 2, N-myristoyltransferase, and N-myristoyltransferase inhibitor protein 71 in HT29

Biochemical and Biophysical Research Communications
Ponniah SelvakumarRajendra K Sharma

Abstract

Protein myristoylation is a co-translational process, catalyzed by N-myristoyltransferase (NMT) that occurs after the initiating methionine is removed by methionine aminopeptidase (MetAP). The enzymes NMT and MetAP play a major role in the process of myristoylation of oncoproteins including the c-src family. In this study, we examined the levels of expression of MetAP2, NMT, and NMT inhibitor protein 71 (NIP71) in human colon cancer cell lines (HCCLs). We examined the influence of cell density on the expression of the above proteins in HT29 cells. Western blot analysis of MetAP2 and NMT demonstrated higher levels of protein expression in low density of HT29 while low expression in high density was observed. In addition, we observed that NIP71 and pp60(c-src) expressions were dependent on the cell density of HT29. This is the first study demonstrating the expression of MetAP2, NMT, pp60(c-src), and NIP71 in HCCLs.

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Citations

Feb 10, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Sanghamitra MitraRichard C Holz
Aug 27, 2005·Molecular Cancer Research : MCR·Charles E DuckerCharles D Smith
Sep 26, 2006·Canadian Journal of Physiology and Pharmacology·Ponniah Selvakumar, Rajendra K Sharma
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Nov 13, 2007·Progress in Neurobiology·Ashakumary LakshmikuttyammaRajendra K Sharma
Jul 24, 2008·International Journal of Cancer. Journal International Du Cancer·Ki-Tae HwangDong-Young Noh
Jan 3, 2006·Biochimica Et Biophysica Acta·Ponniah SelvakumarRajendra K Sharma
Sep 1, 2005·Biochemical and Biophysical Research Communications·Ponniah SelvakumarRajendra K Sharma
Aug 26, 2009·Molecular Cancer·Ponniah SelvakumarRajendra K Sharma
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Feb 14, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Juan A MarchalMaria A Garcia
Sep 1, 2009·Biochimica Et Biophysica Acta·Bansidhar Datta

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