PMID: 8978750Jan 1, 1997Paper

Expression of microtubule-associated protein-2a and other novel microtubule-associated protein-2 transcripts in human fetal spinal cord

Journal of Neurochemistry
N KalchevaB Shafit-Zagardo

Abstract

In human fetal spinal cord (HFSC), six additional microtubule-associated protein-2 (MAP-2) transcripts are generated by alternative splicing of two recently described exons, exon 8 and exon 13. The following three translated proteins are detected by western blot analysis: MAP-2b expressing exon 8 (MAP-2b + 8; MAP-2a), MAP-2b expressing exon 13 (MAP-2b + 13), and MAP-2c expressing exon 8 and exon 13 (MAP-2c + 8 + 13). The finding that MAP-2b + 8 is expressed in HFSC demonstrates for the first time the presence of MAP-2a in human fetal CNS. Immunocytochemical studies show that exon 8-specific antibody and exon 13-specific antibody stain independent and overlapping populations of neurons in the lumbar region of the HFSC. Antibody 13-immunopositive neurons have predominantly cytosolic staining, whereas in the antibody 8-immunoreactive neurons staining was observed in the cytosol, dendrites, and some synapses. The prenatal expression of MAP-2a, which has been used as a marker of synaptogenesis, not only demonstrates the presence of a mature MAP-2 isoform in HFSC, but suggests that MAP-2a is important during human fetal as well as postnatal synaptogenesis.

Citations

May 25, 2002·Journal of Neuropathology and Experimental Neurology·Satoshi O SuzukiBridget Shafit-Zagardo
Dec 20, 2007·Journal of Neuropathology and Experimental Neurology·Bin LiInge Grundke-Iqbal
May 20, 1998·Molecular Neurobiology·B Shafit-Zagardo, N Kalcheva
May 14, 2008·Cell Motility and the Cytoskeleton·Carole Abi Farah, Nicole Leclerc

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