Expression of migration-related genes is progressively upregulated in murine Lineage-Sca-1+c-Kit+ population from the fetal to adult stages of development.

Stem Cell Research & Therapy
Jesús Ciriza, Marcos E García-Ojeda

Abstract

Hematopoietic stem cells (HSCs) follow a genetically programmed pattern of migration during development. Extracellular matrix and adhesion molecules, as well as chemokines and their receptors, are important in adult HSC migration. However, little is known about the role these molecules play at earlier developmental stages. We have analyzed by quantitative polymerase chain reaction (qPCR) array the expression pattern of extracellular matrix and adhesion molecules as well as chemokines and chemokine receptors in Lineage-Sca-1+c-Kit+ (LSK) cells at different stages of development, in order to characterize the role played by these molecules in LSK. Data were represented by volcano plots to show the differences in expression pattern at the time points studied. Our results show marked changes in the expression pattern of extracellular matrix, adhesion molecules, chemokines and their receptors with developmental age, particularly in later stages of development. Ten molecules were significantly increased among the LSK populations studied. Our screen identified the upregulation of Col4a1, as well as molecules involved in its degradation (Mmp2, Timp2), with development. Other genes identified were Sell, Tgfbi, and Entpd1. Furthermore, we...Continue Reading

References

Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·S J MorrisonI L Weissman
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·T PapayannopoulouN S Wolf
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·I GodinA Cumano
Jan 1, 1995·Annual Review of Cell and Developmental Biology·S J MorrisonI L Weissman
Feb 1, 1997·Transplantation Proceedings·H TaniguchiH Nakauchi
Feb 26, 1999·Bone Marrow Transplantation·R BellucciP de Fabritiis
Sep 26, 2001·The Journal of Biological Chemistry·G A McQuibbanC M Overall
Dec 1, 2001·Science·D E WrightI L Weissman
May 8, 2002·The Journal of Experimental Medicine·Douglas E WrightIrving L Weissman
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ekaterina MorgunovaKarl Tryggvason
Sep 14, 2002·Science·Natalia B IvanovaIhor R Lemischka
Oct 29, 2002·Cytokine & Growth Factor Reviews·Patricia MentenJo Van Damme
Dec 4, 2003·Molecular and Cellular Biology·Linda M ScottThalia Papayannopoulou
Mar 17, 2004·PLoS Biology·Julie L ChristensenIrving L Weissman
Jun 17, 2005·Annals of the New York Academy of Sciences·Pierre Charbord, Kateri Moore
Sep 10, 2005·PLoS Genetics·E Camilla ForsbergIrving L Weissman
Feb 24, 2006·Nature Reviews. Immunology·Anne Wilson, Andreas Trumpp
Aug 31, 2006·The Pharmacogenomics Journal·J J ChenC-J Lin
Sep 14, 2006·Development·Hanna K A Mikkola, Stuart H Orkin
Jan 2, 2007·Trends in Molecular Medicine·Rosandra N KaplanDavid Lyden
Apr 20, 2007·Annals of the New York Academy of Sciences·Anne WilsonAndreas Trumpp
Apr 27, 2007·Nature·Hiroo Ueno, Irving L Weissman
Dec 18, 2007·Annals of the New York Academy of Sciences·Simón Méndez-Ferrer, Paul S Frenette
Feb 26, 2008·Cell·Diana J LairdAmy J Wagers
Mar 29, 2008·Cell Stem Cell·Andrea T HooperShahin Rafii

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Citations

Sep 8, 2012·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Catherine GodfraindHarry Kozakewich
Nov 13, 2010·Cancer Prevention Research·Seetharaman BalasenthilAnn McNeill Killary
Nov 30, 2011·TheScientificWorldJournal·Daisuke SugiyamaYuka Horio
Jun 27, 2013·Experimental Hematology·Huimin CaoSusan K Nilsson
Mar 26, 2011·Journal of Cellular Physiology·Luciana Doria GuarinielloSergio Tufik
Oct 13, 2017·European Journal of Immunology·Andrea CossarizzaJakob Zimmermann
Apr 25, 2020·International Journal of Molecular Sciences·Federica BarbagalloManuela Pellegrini
Aug 6, 2020·Frontiers in Cell and Developmental Biology·Rohan Kulkarni, Vaijayanti Kale
Aug 17, 2019·Fish & Shellfish Immunology·Armando Vega-LópezElba Reyes-Maldonado
Jan 26, 2021·Journal of Cellular Physiology·Dushyant Kumar GautamIndira Paddibhatla

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Methods Mentioned

BETA
flow cytometry
PCR

Software Mentioned

RT 2 Profiler PCR Array Data Analysis
BD
FlowJo
SPSS
BD [UNK]

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