Expression of miR-30c and BCL-9 in gastric carcinoma tissues and their function in the development of gastric cancer

Oncology Letters
Wenyan HanXiulan Su

Abstract

microRNA-30c (miR-30c) is a member of the miR-30s family, which is known to serve important roles in the occurrence and development of numerous tumor types. Our previous microarray analysis of extracted RNA from tissue samples was conducted to examine the expression of miR-30c and predict miR-30c target genes. In the present study, it was determined that the expression of miR-30c was differentially expressed in 82 paired gastric cancer (GC) and paracancerous tissues. Cellular expression of miR-30c in two GC cell lines MKN-45, MKN-74 and one non-cancer cell line GES-1 was modified using the miR-30c-mimic and miR-30c-inhibitor reagents, in a series of transfection experiments. Following transfection of cancer and non-cancer cell lines with the miR-30c-mimic, cell proliferation and apoptosis rates were increased. Compared with the NC group, MKN-74 cell proliferation was significantly inhibited (P<0.05) following transfection with the miR-30c-mimic at 48 and 24 h, GES-1 was significantly inhibited (P<0.05) at 24 and 48 h, and apoptosis was significantly reduced in transfected MKN-74 cells (P<0.05). The clinicopathological data and the expression of BCL-9 and miR-30c in patients with GC were used to identify associations. The expres...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 17, 2004·Nature·Victor Ambros
Dec 27, 2005·Current Opinion in Genetics & Development·Felix H BrembeckWalter Birchmeier
Jan 31, 2006·Developmental Biology·Knud NairzErnst Hafen
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefano VoliniaCarlo M Croce
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
May 10, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Farin KamangarWilliam F Anderson
Oct 3, 2006·Developmental Cell·Wigard P Kloosterman, Ronald H A Plasterk
Oct 25, 2006·Nature Reviews. Cancer·George A Calin, Carlo M Croce
Sep 25, 2007·Methods : a Companion to Methods in Enzymology·Bingbing WangCarl D Novina
Feb 22, 2008·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Aug 8, 2008·Bioinformation·Jayapal ManikandanPeter Natesan Pushparaj
Nov 28, 2008·Proceedings of the National Academy of Sciences of the United States of America·Juliusz MieszczanekMariann Bienz
May 6, 2009·Stem Cells·Shigeo TakaishiTimothy C Wang
Jul 25, 2009·Lancet·Henk H HartgrinkCornelis J H van de Velde
Sep 24, 2009·Cancer Research·Douglas R HurstDanny R Welch
Dec 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ivan MartinezDaniel DiMaio
Dec 12, 2012·Breast Cancer Research and Treatment·Jessica BockhornHuiping Liu
Mar 24, 2015·PloS One·Qian ZhangXu Gao

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Methods Mentioned

BETA
chip
transfection
Flow Cytometry
electrophoresis
PCR

Software Mentioned

SPSS
iScan System
Bio
CellSens Standard
Capt
LuxScan image analysis system
Flow Cytometry Modeling

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