Expression of miR-34a in T-Cells Infected by Human T-Lymphotropic Virus 1

Frontiers in Microbiology
Varun K SharmaDonna M D'Agostino

Abstract

Human T-lymphotropic virus 1 (HTLV-1) immortalizes T-cells and is the causative agent of adult T-cell leukemia/lymphoma (ATLL). HTLV-1 replication and transformation are governed by multiple interactions between viral regulatory proteins and host cell factors that remain to be fully elucidated. The present study investigated the impact of HTLV-1 infection on the expression of miR-34a, a microRNA whose expression is downregulated in many types of cancer. Results of RT-PCR assays showed that five out of six HTLV-1-positive cell lines expressed higher levels of miR-34a compared to normal PBMC or purified CD4+ T-cells. ATLL cell line ED, which did not express miR-34a, showed methylation of the miR-34a promoter. Newly infected PBMC and samples from 10 ATLL patients also showed a prominent increase in miR-34a expression compared to PBMC controls. The primary miR-34a transcript expressed in infected cell line C91PL contained binding motifs for NF-κB and p53. Pharmacological inhibition of NF-κB with Bay 11-7082 indicated that this pathway contributes to sustain miR-34a levels in infected cells. Treatment of infected cell lines with the p53 activator nutlin-3a resulted in a further increase in miR-34a levels, thus confirming it as a tra...Continue Reading

References

May 3, 1986·Lancet·M OsameM Tara
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B J PoieszR C Gallo
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M PopovicR C Gallo
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M YoshidaY Hinuma
Nov 21, 2001·The Journal of Biological Chemistry·William H HoffmanMaureen Murphy
Jan 6, 2004·Science·Lyubomir T VassilevEmily A Liu
Feb 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·George Adrian CalinCarlo M Croce
Mar 3, 2004·International Journal of Cancer. Journal International Du Cancer·Satoshi TakedaMasao Matsuoka
Sep 13, 2005·Oncogene·Shao-Cong Sun, Shoji Yamaoka
Sep 30, 2005·Nucleic Acids Research·Jinmai JiangThomas D Schmittgen
Jun 2, 2007·Molecular Cell·Nina Raver-ShapiraMoshe Oren
Jun 2, 2007·Molecular Cell·Tsung-Cheng ChangJoshua T Mendell
Jun 8, 2007·Nature·Lin HeGregory J Hannon
Jul 28, 2007·Current Biology : CB·Guido T BommerEric R Fearon
Sep 19, 2007·International Journal of Hematology·Yorifumi Satou, Masao Matsuoka
Sep 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi TazawaHitoshi Nakagama
Apr 15, 2008·FEBS Letters·Fang SunXiaofei Zheng
May 29, 2008·Molecular Cancer Research : MCR·Kristina A ColeJohn M Maris
Jun 21, 2008·Molecular Cancer Therapeutics·Michela MuscoliniLoretta Tuosto
Aug 23, 2008·Cell Cycle·Dmitri LodyginHeiko Hermeking
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Munekazu YamakuchiCharles J Lowenstein
May 23, 2009·Cell Death and Differentiation·H Hermeking
Jul 25, 2009·Nature·Hiroshi I SuzukiKohei Miyazono
Aug 22, 2009·Cell Death and Differentiation·N R ChristoffersenA H Lund
Sep 24, 2009·Cancer Research·Yunqing LiRoger Abounader
Dec 18, 2009·International Journal of Cancer. Journal International Du Cancer·Mariko TomitaNaoki Mori
Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Munekazu YamakuchiCharles J Lowenstein

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Citations

Dec 15, 2019·Pathogens·Stefania FochiMaria Grazia Romanelli
Nov 30, 2019·Oxidative Medicine and Cellular Longevity·Francesco CiccareseStefano Indraccolo
Dec 22, 2020·Neurobiology of Stress·Li-Tao YiQing Liu
Aug 9, 2020·Life Sciences·Forough TaheriSomayeh Reiisi
May 28, 2019·Journal of Autoimmunity·Mengxiao XieQixiang Shao

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Methods Mentioned

BETA
flow cytometry
PCR
transfection
acetylation
protein assay
transfections

Software Mentioned

Finch TV
ModFit
Geospiza

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