Expression of miR-634 in gastric carcinoma and its effects on proliferation, migration, and invasion of gastric cancer cells.

Cancer Medicine
Jiao GuoDong-Qiu Dai

Abstract

This study aims to observe the expression of microRNA (miR)-634 in different gastric cancer cell lines and tissues, and to study the effects of miR-634 on the proliferation, migration, and invasion of the gastric cancer cells. The miR-634 mimics and miR-634 inhibitors were transfected by lentivirus into human gastric cancer SGC-7901 and MGC-803 cells, and the miR-634 cells without transfection were used as the control group (NC group). The expression of miR-634 in the transfected cells was detected by qRT-PCR. Cell viability was measured by the CCK8 assay. The migration and invasion ability of the cells were detected by scratch assays and Transwell® chamber assays, respectively, and the luciferase assay verified the binding of miR-634 to the target gene JAG1. The expression level of miR-634 in gastric cancer tissues and cell lines was significantly lower than that in normal adjacent tissues and control cells. The survival of cells was significantly decreased, and number of cells migrating and invading was decreased in the miR-634 mimics group. However, in the miR-634 inhibitor group, the opposite results were observed. Over-expression of miR-634 inhibited the proliferation, migration, and invasion of gastric cancer cell lines, ...Continue Reading

References

Feb 24, 2011·Cancer Research·Päivi ÖstlingOlli Kallioniemi
May 3, 2011·Digestive Diseases and Sciences·Xiaoli LiJiansheng Li
Oct 24, 2013·Molecular Oncology·Suvi-Katri LeivonenMerja Perälä
Oct 14, 2014·Frontiers in Oncology·Demin LiAdrian L Harris
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Feb 24, 2015·Pathology, Research and Practice·Jiayu LiDaoyuan Si
Sep 15, 2015·Artificial Cells, Nanomedicine, and Biotechnology·Jianglin CongYouzhong Zhang
Nov 19, 2015·Molecular Cancer·Marijn T M van JaarsveldErik A C Wiemer
May 31, 2016·World Journal of Gastroenterology : WJG·Erin Jou, Lakshmi Rajdev
Jul 30, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yantao DuLiankun Gu
Oct 4, 2016·Molecular Oncology·Chris Zhiyi ZhangMei-Fang Zhang
Dec 17, 2016·Cancer Letters·João Ramalho-CarvalhoCarmen Jerónimo
Feb 17, 2017·Scientific Reports·Hai-Ting LiuPeng Gao
Mar 16, 2017·World Journal of Biological Chemistry·Stefania OlivetoStefano Biffo

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection
flow cytometry
protein concentration assay
transfections

Software Mentioned

SPSS
Methprimer
TargetScan
miRanda
Methly Primer

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.