Expression of miRNA-143 in Pancreatic Cancer and Its Clinical Significance

Cancer Biotherapy & Radiopharmaceuticals
Bo XuLiang Lai

Abstract

To analyze the expression of micro-RNA 143 (miRNA-143) in the patients with pancreatic cancer and to explore the influence of overexpression of miRNA-143 on pancreatic cancer cells. Twenty-five patients with pancreatic cancer that received treatment in their hospital were included in this study. Pancreatic cancer tissues of the patients were surgically removed, and cancer-adjacent tissues were taken for control. Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expressions of miRNA-143 and its target gene Kirsten rat sarcoma (KRAS) in pancreatic cancer tissues and cancer-adjacent tissues. Western blot test was conducted to detect the expression of KRAS in pancreatic cancer tissues. Clinicopathologic data of patients were recorded in detail, and the analysis on the correlation of these data with the expression of miRNA-143 in pancreatic cancer tissues was conducted. Pancreatic cancer cell line (PANC-1) was transfected with plasmid for overexpression of miRNA-143, and the influences of miRNA-143 on the apoptosis, migration ability, and invasion ability of the cells were verified by flow cytometry, cell scratch experiment, and Transwell experiment. The results of semiquantitative RT-P...Continue Reading

References

Dec 7, 2013·World Journal of Gastroenterology : WJG·Raffaele PezzilliUNKNOWN Exocrine Pancreatic Insufficiency collaborative (EPIc) Group
Feb 26, 2015·Oncotarget·Gregory M Heestand, Razelle Kurzrock
Apr 24, 2015·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Li LiYing-zhi Qin
Feb 6, 2016·Journal of Experimental & Clinical Cancer Research : CR·Li HuangXingpeng Wang
Jun 9, 2016·Diagnostic Pathology·Wenzel M HackengLodewijk A A Brosens

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Citations

Mar 3, 2020·Journal of Toxicology and Environmental Health. Part a·Kihun KimYun Hak Kim
Aug 25, 2020·Gastroenterology Research and Practice·Gongpan LiuJiangong Li
Jul 29, 2020·Antioxidants·Anita ThyagarajanRavi P Sahu
Dec 20, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Bin XuJunfei Shao

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Methods Mentioned

BETA
PCR
electrophoresis
transfection
reverse transcription-PCR
Flow cytometry

Software Mentioned

Statistical Product and Service Solutions ( SPSS )
ImageJ

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