Expression of mitochondria-dependent apoptosis genes (p53, Bax, and Bcl-2) in rat granulosa cells during follicular development

Journal of the Society for Gynecologic Investigation
DooSeok ChoiDukSoo Bae

Abstract

We examined rat ovarian granulosa cells at different follicular stages and evaluated the apoptosis pattern of the mitochondria-dependent genes during folliculogenesis. After down-regulating ovarian function with gonadotropin-releasing hormone agonist (GnRHa), granulosa cells were collected from the rat ovary at different stages of the following different hormonal treatment paradigms: stage E (after estrogen treatment), EF (after E + follicle-stimulating hormone [FSH] treatment), and EF hCG (after E + FSH + human chorionic gonadotropin treatment). To evaluate the in vitro susceptibility of granulosa cells at different developmental stages to apoptosis, the collected cells were cultured in a serum-free medium with or without E2 for 24 hours. The regulation of apoptosis in the granulosa cells was analyzed using fluorescein-activated cell sorting, quantitative competitive polymerase chain reaction, and western blot methods. The apoptosis rate of the freshly isolated granulosa cells tended to increase according to the hormonal treatment paradigm. In addition, during the hormone treatment, mitochondria-dependent apoptosis genes showed the following changes: although the Bax mRNA level did not change, the Bcl-2 mRNA level decreased si...Continue Reading

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Citations

Jul 15, 2015·Reproductive Medicine and Biology·Yuri YamamotoMinoru Irahara
Mar 17, 2017·Zygote : the Biology of Gametes and Early Embryos·Ellen de Vasconcelos da CunhaJosé Roberto Viana Silva
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Aug 6, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Kimberly P MillerJodi A Flaws
Oct 2, 2019·Molecular Reproduction and Development·Ghassan M A IshakEduardo L Gastal
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Zahra Ranjbar-NavaziSoodabeh Davaran
Apr 27, 2021·Journal of Animal Physiology and Animal Nutrition·Taojie ZhangSheng Cui
Jul 20, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Pengju ZhangXinghong Wu

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