PMID: 20635039Jul 17, 2010Paper

Expression of MMP-2 during differentiation of vascular endothelial cell from bone marrow mesenchymal stem cell in porcine

Shanghai kou qiang yi xue = Shanghai journal of stomatology
Li-jun FangQiu-xu Wang

Abstract

To investigate the expression of MMP-2 in porcine bone marrow mesenchymal stem cells (MSCs) in the process of differentiation into vascular endothelial cells in vitro. Porcine MSCs were isolated from bone marrow and grown in vitro. At 3-, 7-day after VEGF induction, immunohistochemical and flow cytometrical examinations were carried out to detect the staining of FVIII. The expression of MMP-2 was analyzed by Western blot. At 3-, 7-day after VEGF induction, the morphology of the cells gradually changed. The cells gradually showed a ball-like appearance at 3-day. They connected with adjacent cells and formed tube-like structure after 7 days. The factor VIII-positive cells could be observed at 3-day after VEGF induction. The positive cells increased and joined to neighboring FVIII-positive cells, formed tube-like structures at 7-day after VEGF treatment. MSCs maintained in control medium stained negative. FACS analysis showed that untreated MSCs were negative for FVIII. The number of FVIII positive cells was not significantly increased at 3-day after induction (3%). But at 7-day after induction, FVIII-positive cells were significantly increased (13%). The expression of MMP-2 was increased as early as 1 hour post VEGF treatment. Po...Continue Reading

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