PMID: 11341602May 9, 2001Paper

Expression of molecular messages for angiogenesis by fibroblasts from aneurysmal abdominal aorta versus dermal fibroblasts

International Journal of Surgical Investigation
M D TilsonT McCaffrey

Abstract

The molecular messages which drive angiogenesis in the adventitia of an aneurysmal aorta are uncertain. The emergence of molecular phenotyping by cDNA expression arrays provides a simple and rapid method for a preliminary approach to the analysis of molecular messengers for neovascularization in cultured cells. In the present experiment, fibroblasts cultured from the aorta of a patient with abdominal aortic aneurysm (AAA) were compared with normal dermal fibroblasts, on an array that evaluates several mRNAs with known roles in angiogenesis. RNA was isolated from fibroblasts and purified. Labelled cDNA probes were generated from a mixture of RNA and CDS primers. Atlas Array membranes (Clontech) were prehybridized by ExpressHyb buffer. The cDNA probes were then added to the membranes, which were exposed to Phospholmage Screen (Molecular Dynamics) and analysed by a dedicated computer program. The most significantly upregulated mRNAs in AAA (by comparison to dermal fibroblasts) were: MCAF, MDNCF, EGR-1, VEGF, FGF-7, Mal protein, Mac Marcks, Transducin, Interleukin-9 receptor, and TNF. VEGF and TNF were upregulated, as expected. However, the upregulation of monocyte chemotactic and activating factor (MCAF) and monocyte-derived neutr...Continue Reading

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Aortic Aneurysm

An aortic aneurysm is the weakening and bulging of the blood vessel wall in the aorta. This causes dilatation of the aorta, which is usually asymptomatic but carries the risk of rupture and hemorrhage. Find the latest research on aortic aneurysms here.

Aneurysm

Aneurysms are outward distensions or bulges that occurs in a weakened wall of blood vessels. Discover the latest research on aneurysms here.