PMID: 15329480Aug 27, 2004Paper

Expression of mRNA for the angiopoietin-tie system in granulosa cells during follicular development in cows

The Journal of Reproduction and Development
Ken-Go HayashiA Miyamoto

Abstract

Recent findings indicate that the changing profile of angiopoietins (ANPT) and their receptor Tie2 are closely associated with development and regression of the vascular network in the cyclic ovary. We previously reported that mRNA expression for the ANPT-Tie system in theca interna changes during bovine follicular development and atresia, and both ANPTs affect steroidogenesis in the preovulatory follicle. The aim of this study was to investigate mRNA expression for ANPT1, ANPT-2 and Tie2 in granulosa cells (GC) during follicular development in the cow. Bovine follicles were classified according to the estradiol-17beta (E(2)) concentration in follicular fluid (FF) as follows: (1) E(2)<0.5, (2) 0.5<E(2)<5, (3) 5<E(2)<20, (4) 20<E(2)<180 and (5) E(2)>180 ng/ml FF. Semi-quantitative RT-PCR analysis revealed that the expression of ANPT-1 mRNA was not detected in most of the follicle with E(2)<5 ng/ml (diameter of 5-10 mm), but clearly detected in all follicles with E(2)>5 ng/ml (diameter of >10 mm). The mRNA expression for ANPT-2 was drastically decreased in the follicles with E(2)>5 ng/ml. Tie2 mRNA expression remained unchanged at the different stages of follicular development. The present data show that ANPT-1 becomes predominan...Continue Reading

References

Feb 1, 1989·European Journal of Obstetrics, Gynecology, and Reproductive Biology·D Kranzfelder, B Maurer-Schultze
Jul 1, 1982·Journal of Reproduction and Fertility·K M HendersonI A Swanston
Jul 1, 1994·Journal of Animal Science·M F SmithG W Smith
Sep 23, 2000·Nature·G D YancopoulosJ Holash
Dec 26, 2001·Archives of Medical Research·R L StoufferT M Hazzard

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Citations

Sep 30, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·R S RobinsonG E Mann
Dec 8, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Yung-Sen HuangNeng-Lang Shih
Jul 7, 2005·Domestic Animal Endocrinology·B Berisha, D Schams
Jun 10, 2005·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Kanako MiyabayashiEimei Sato
Sep 2, 2020·Reproduction in Domestic Animals = Zuchthygiene·Bajram BerishaMichael W Pfaffl
Mar 27, 2021·Frontiers in Physiology·Sina StuckerAnjali P Kusumbe

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