PMID: 11930709Apr 5, 2002Paper

Expression of mRNA of osteocalcin in condylar cartilage of young SD rats after functional protrusion

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology
L YeS Luo

Abstract

To study the temporal and spatial patterns of the expression of mRNA in condylar cartilage of young growing SD rats, in condition of different functional treatment time span. SD rats are randomly divided into 3 groups: control, all-day group (wearing functional appliance for 24 hours a day) and 12-hour group (wearing appliance for 12 hours in daytime). After one week, animals are sacrificed and Osteocalcin mRNA expression is studied by in situ hybridization using Oligonucleotide probe, calculated by image processing and macroscopic and microscopic statistics. Osteocalcin mRNA is expressed in condylar cartilage of young growing SD rats and the acrophase is around 6:00 am. After wearing the functional appliance, the intensity in all-day group rises more than that in 12-hourgroup. Better results can be achieved in all-day group with functional appliance.

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